The preparation of size-controlled functionalized polymeric nanoparticles in micelles.

The reverse micellar system of dioctyl-sulfosuccinate (AOT)/octane and toluene have been used as a template for polymerization of acrylamide (AA)/bisacrylamide (BAA)-based functionalized polymeric nanoparticles. Such nanoparticles are typically sized between 20 and 90 nm. They can be synthesized with different functional groups according to the monomers added to the polymerization mixture. In our experiments the nanoparticles carried amino and carboxyl groups following incorporation of allylamine (AAm) or methacrylic acid (MAA) monomers, respectively. The available amine or carboxyl groups can then be used for immobilization of enzymes or other biomolecules. These enzymes, subtilisin, laccase and lipase, were immobilized onto polyAA/BAA/MAA nanoparticles covalently after activating the MAA carboxylic groups with Woodward's K reagent. Non-covalent immobilization via electrostatic interaction was also performed.