Literature DB >> 19563831

Flow cytometric quantification of chlamydial infection in cell culture.

Michael Grün1, Marita Liebisch, Helga Sauerwein, Gerhard Jahreis, Konrad Sachse.   

Abstract

A flow cytometric method was developed, which allows fast and efficient analysis of cell cultures infected with chlamydiae. The proportion of positive cells increased with the infectious dose and correlated with chlamydia copy numbers calculated from real-time PCR. While retaining the advantages of single-cell analysis, flow cytometry allows handling of large sample numbers and counterstaining for additional marker proteins.

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Year:  2009        PMID: 19563831     DOI: 10.1016/j.mimet.2009.06.016

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  4 in total

1.  Chlamydia trachomatis immune evasion via downregulation of MHC class I surface expression involves direct and indirect mechanisms.

Authors:  Joyce A Ibana; Danny J Schust; Jun Sugimoto; Takeshi Nagamatsu; Sheila J Greene; Alison J Quayle
Journal:  Infect Dis Obstet Gynecol       Date:  2011-05-29

2.  Loss of FADS2 function severely impairs the use of HeLa cells as an in vitro model for host response studies involving fatty acid effects.

Authors:  Anke Jaudszus; Christian Degen; Stephan W Barth; Martin Klempt; Wiebke Schlörmann; Alexander Roth; Carsten Rohrer; Helga Sauerwein; Konrad Sachse; Gerhard Jahreis
Journal:  PLoS One       Date:  2014-12-30       Impact factor: 3.240

3.  A simple, fast and reliable scan-based technique as a novel approach to quantify intracellular bacteria.

Authors:  Meysam Sarshar; Daniela Scribano; Giulia Tranquilli; Marisa Di Pietro; Simone Filardo; Carlo Zagaglia; Rosa Sessa; Anna Teresa Palamara; Cecilia Ambrosi
Journal:  BMC Microbiol       Date:  2019-11-12       Impact factor: 3.605

4.  A Novel Flow Cytometric Approach for the Quantification and Quality Control of Chlamydia trachomatis Preparations.

Authors:  Romana Klasinc; Michael Reiter; Astrid Digruber; Waltraud Tschulenk; Ingrid Walter; Alexander Kirschner; Andreas Spittler; Hannes Stockinger
Journal:  Pathogens       Date:  2021-12-12
  4 in total

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