Literature DB >> 19557354

Antibacterial activity of recombinant hCAP18/LL37 protein secreted from Pichia pastoris.

Soon-ja Kim1, Renshu Quan, Sung-Jin Lee, Hak-Kyo Lee, Joong-Kook Choi.   

Abstract

Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZaA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.

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Year:  2009        PMID： 19557354     DOI： 10.1007/s12275-009-0131-9

Source DB:  PubMed          Journal:  J Microbiol        ISSN： 1225-8873            Impact factor:   3.422


  29 in total

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Authors:  Sue Macauley-Patrick; Mariana L Fazenda; Brian McNeil; Linda M Harvey
Journal:  Yeast       Date:  2005-03       Impact factor: 3.239

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Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

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Journal:  J Biol Chem       Date:  1988-11-15       Impact factor: 5.157

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  4 in total

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