AIMS: To investigate spontaneous polymorphonuclear neutrophils (PMNs) apoptosis in unstable angina (UA) and its association with recurrence of instability. METHODS AND RESULTS: We compared PMNs apoptotic rate at 4 and 24 h in patients with UA, stable angina (SA), and controls (H) with two different protocols by flow cytometry. We measured apoptotic rate of isolated PMNs (Protocol 1) in 30 UA patients, 13 SA patients, and 34 H; and apoptosis of PMNs in whole blood culture (Protocol 2) in further 10 UA patients, 7 SA patients, and 6 H. Serum high-sensitivity C-reactive protein was also measured. Polymorphonuclear neutrophils of UA patients showed a decreased apoptotic rate compared with SA patients and H at 4 h in Protocol 1 (both P < 0.01), and at 24 h in Protocol 2 (P < 0.05 and <0.01, respectively). In overall population, a negative correlation was found between apoptotic rate at 4 h and high-sensitivity C-reactive protein levels (P < 0.01). Six among 40 patients with UA had early recurrence of symptoms and their apoptotic rate was significantly reduced compared with UA patients without recurrence of symptoms (P = 0.024). CONCLUSIONS: Our study demonstrates delayed PMN apoptosis in UA. This alteration might be involved in the persistence of inflammatory activation and affects recurrence of instability.
AIMS: To investigate spontaneous polymorphonuclear neutrophils (PMNs) apoptosis in unstable angina (UA) and its association with recurrence of instability. METHODS AND RESULTS: We compared PMNs apoptotic rate at 4 and 24 h in patients with UA, stable angina (SA), and controls (H) with two different protocols by flow cytometry. We measured apoptotic rate of isolated PMNs (Protocol 1) in 30 UA patients, 13 SA patients, and 34 H; and apoptosis of PMNs in whole blood culture (Protocol 2) in further 10 UA patients, 7 SA patients, and 6 H. Serum high-sensitivity C-reactive protein was also measured. Polymorphonuclear neutrophils of UA patients showed a decreased apoptotic rate compared with SA patients and H at 4 h in Protocol 1 (both P < 0.01), and at 24 h in Protocol 2 (P < 0.05 and <0.01, respectively). In overall population, a negative correlation was found between apoptotic rate at 4 h and high-sensitivity C-reactive protein levels (P < 0.01). Six among 40 patients with UA had early recurrence of symptoms and their apoptotic rate was significantly reduced compared with UA patients without recurrence of symptoms (P = 0.024). CONCLUSIONS: Our study demonstrates delayed PMN apoptosis in UA. This alteration might be involved in the persistence of inflammatory activation and affects recurrence of instability.
Authors: Roberta Della Bona; Maria Teresa Cardillo; Milena Leo; Gina Biasillo; Massimo Gustapane; Francesco Trotta; Luigi M Biasucci Journal: Inflamm Res Date: 2013-04-03 Impact factor: 4.575
Authors: Ilze Bot; Isabelle T M N Daissormont; Alma Zernecke; Gijs H M van Puijvelde; Birgit Kramp; Saskia C A de Jager; Judith C Sluimer; Marco Manca; Veronica Hérias; Marijke M Westra; Martine Bot; Peter J van Santbrink; Theo J C van Berkel; Lishan Su; Mona Skjelland; Lars Gullestad; Johan Kuiper; Bente Halvorsen; Paul Aukrust; Rory R Koenen; Christian Weber; Erik A L Biessen Journal: J Mol Cell Cardiol Date: 2014-05-08 Impact factor: 5.000
Authors: Julian I Borissoff; Ivo A Joosen; Mathijs O Versteylen; Alexander Brill; Tobias A Fuchs; Alexander S Savchenko; Maureen Gallant; Kimberly Martinod; Hugo Ten Cate; Leonard Hofstra; Harry J Crijns; Denisa D Wagner; Bas L J H Kietselaer Journal: Arterioscler Thromb Vasc Biol Date: 2013-07-01 Impact factor: 8.311
Authors: Durmuş Yıldıray Sahin; Mustafa Gür; Zafer Elbasan; Ali Yıldız; Zekeriya Kaya; Yahya Kemal Içen; Ali Kıvrak; Caner Türkoğlu; Remzi Yılmaz; Murat Caylı Journal: Exp Clin Cardiol Date: 2013