Literature DB >> 19554299

Expression of single-chain Fv fragments in E. coli cytoplasm.

Laurence Guglielmi1, Pierre Martineau.   

Abstract

The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished by using either specially engineered E. coli strains or hyperstable scFvs.

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Year:  2009        PMID： 19554299      PMCID： PMC3101525          DOI： 10.1007/978-1-60327-302-2_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

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