Literature DB >> 19554297

Intrabody expression in eukaryotic cells.

Laurence Guglielmi1, Pierre Martineau.   

Abstract

We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.

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Year:  2009        PMID： 19554297      PMCID： PMC3194088          DOI： 10.1007/978-1-60327-302-2_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras.

Authors:  M Lener; I R Horn; A Cardinale; S Messina; U B Nielsen; S M Rybak; H R Hoogenboom; A Cattaneo; S Biocca
Journal:  Eur J Biochem       Date:  2000-02

Review 2.  Stability engineering of antibody single-chain Fv fragments.

Authors:  A Wörn; A Plückthun
Journal:  J Mol Biol       Date:  2001-02-02       Impact factor: 5.469

3.  De novo production of diverse intracellular antibody libraries.

Authors:  Tomoyuki Tanaka; Grace T Y Chung; Alan Forster; M Natividad Lobato; Terence H Rabbitts
Journal:  Nucleic Acids Res       Date:  2003-03-01       Impact factor: 16.971

4.  Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework.

Authors:  Adrian Auf der Maur; Christian Zahnd; Franziska Fischer; Silvia Spinelli; Annemarie Honegger; Christian Cambillau; Dominik Escher; Andreas Plückthun; Alcide Barberis
Journal:  J Biol Chem       Date:  2002-09-04       Impact factor: 5.157

5.  Validation of a stable recombinant antibodies repertoire for the direct selection of functional intracellular reagents.

Authors:  Maria Elena Villani; Mariasole Di Carli; Marcello Donini; Giorgio Traversini; Chiara Lico; Rosella Franconi; Eugenio Benvenuto; Angiola Desiderio
Journal:  J Immunol Methods       Date:  2007-10-01       Impact factor: 2.303

Review 6.  Intracellular antibodies and challenges facing their use as therapeutic agents.

Authors:  M Natividad Lobato; Terence H Rabbitts
Journal:  Trends Mol Med       Date:  2003-09       Impact factor: 11.951

7.  The mode of action of Y13-259 scFv fragment intracellularly expressed in mammalian cells.

Authors:  A Cardinale; M Lener; S Messina; A Cattaneo; S Biocca
Journal:  FEBS Lett       Date:  1998-11-20       Impact factor: 4.124

8.  A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm.

Authors:  Pascal Philibert; Audrey Stoessel; Wei Wang; Annie-Paule Sibler; Nicole Bec; Christian Larroque; Jeffery G Saven; Jérôme Courtête; Etienne Weiss; Pierre Martineau
Journal:  BMC Biotechnol       Date:  2007-11-22       Impact factor: 2.563
  8 in total
  3 in total

Review 1.  Antibodies Targeting the Transferrin Receptor 1 (TfR1) as Direct Anti-cancer Agents.

Authors:  Pierre V Candelaria; Lai Sum Leoh; Manuel L Penichet; Tracy R Daniels-Wells
Journal:  Front Immunol       Date:  2021-03-17       Impact factor: 7.561

2.  Simple Fluorogenic Cellular Assay for Histone Deacetylase Inhibitors Based on Split-Yellow Fluorescent Protein and Intrabodies.

Authors:  Yuki Ohmuro-Matsuyama; Tetsuya Kitaguchi; Hiroshi Kimura; Hiroshi Ueda
Journal:  ACS Omega       Date:  2021-04-07

Review 3.  Single-Chain Fragment Variable: Recent Progress in Cancer Diagnosis and Therapy.

Authors:  Paola Muñoz-López; Rosa María Ribas-Aparicio; Elayne Irene Becerra-Báez; Karla Fraga-Pérez; Luis Fernando Flores-Martínez; Armando Alfredo Mateos-Chávez; Rosendo Luria-Pérez
Journal:  Cancers (Basel)       Date:  2022-08-30       Impact factor: 6.575
  3 in total

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