Literature DB >> 19553390

Synergism between the components of the bipartite major immediate-early transcriptional enhancer of murine cytomegalovirus does not accelerate virus replication in cell culture and host tissues.

Kai A Kropp1, Christian O Simon1, Annette Fink1, Angélique Renzaho1, Birgit Kühnapfel1, Jürgen Podlech1, Matthias J Reddehase1, Natascha K A Grzimek1.   

Abstract

Major immediate-early (MIE) transcriptional enhancers of cytomegaloviruses are key regulators that are regarded as determinants of virus replicative fitness and pathogenicity. The MIE locus of murine cytomegalovirus (mCMV) shows bidirectional gene-pair architecture, with a bipartite enhancer flanked by divergent core promoters. Here, we have constructed recombinant viruses mCMV-DeltaEnh1 and mCMV-DeltaEnh2 to study the impact of either enhancer component on bidirectional MIE gene transcription and on virus replication in cell culture and various host tissues that are relevant to CMV disease. The data revealed that the two unipartite enhancers can operate independently, but synergize in enhancing MIE gene expression early after infection. Kick-start transcription facilitated by the bipartite enhancer configuration, however, did not ultimately result in accelerated virus replication. We conclude that virus replication, once triggered, proceeds with a fixed speed and we propose that synergism between the components of the bipartite enhancer may rather increase the probability for transcription initiation.

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Year:  2009        PMID： 19553390     DOI： 10.1099/vir.0.012245-0

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  12 in total

1.  Enhancerless cytomegalovirus is capable of establishing a low-level maintenance infection in severely immunodeficient host tissues but fails in exponential growth.

Authors:  Jürgen Podlech; Rares Pintea; Kai A Kropp; Annette Fink; Niels A W Lemmermann; Katja C Erlach; Elena Isern; Ana Angulo; Peter Ghazal; Matthias J Reddehase
Journal:  J Virol       Date:  2010-04-07       Impact factor: 5.103

Review 2.  Viral latency drives 'memory inflation': a unifying hypothesis linking two hallmarks of cytomegalovirus infection.

Authors:  Christof K Seckert; Marion Griessl; Julia K Büttner; Sabine Scheller; Christian O Simon; Kai A Kropp; Angélique Renzaho; Birgit Kühnapfel; Natascha K A Grzimek; Matthias J Reddehase
Journal:  Med Microbiol Immunol       Date:  2012-09-19       Impact factor: 3.402

Review 3.  Principles for studying in vivo attenuation of virus mutants: defining the role of the cytomegalovirus gH/gL/gO complex as a paradigm.

Authors:  Jürgen Podlech; Matthias J Reddehase; Barbara Adler; Niels A W Lemmermann
Journal:  Med Microbiol Immunol       Date:  2015-03-18       Impact factor: 3.402

4.  An endocytic YXXΦ (YRRF) cargo sorting motif in the cytoplasmic tail of murine cytomegalovirus AP2 'adapter adapter' protein m04/gp34 antagonizes virus evasion of natural killer cells.

Authors:  Annette Fink; Franziska Blaum; Marina Babic Cac; Stefan Ebert; Niels A W Lemmermann; Matthias J Reddehase
Journal:  Med Microbiol Immunol       Date:  2015-04-08       Impact factor: 3.402

Review 5.  Refining human T-cell immunotherapy of cytomegalovirus disease: a mouse model with 'humanized' antigen presentation as a new preclinical study tool.

Authors:  Niels A W Lemmermann; Matthias J Reddehase
Journal:  Med Microbiol Immunol       Date:  2016-08-18       Impact factor: 3.402

6.  Reversible inhibition of murine cytomegalovirus replication by gamma interferon (IFN-γ) in primary macrophages involves a primed type I IFN-signaling subnetwork for full establishment of an immediate-early antiviral state.

Authors:  Kai A Kropp; Kevin A Robertson; Garwin Sing; Sara Rodriguez-Martin; Mathieu Blanc; Paul Lacaze; Muhamad F B Noor Hassim; Mizanur R Khondoker; Andreas Busche; Paul Dickinson; Thorsten Forster; Birgit Strobl; Mathias Mueller; Stipan Jonjic; Ana Angulo; Peter Ghazal
Journal:  J Virol       Date:  2011-07-20       Impact factor: 5.103

7.  Stochastic Episodes of Latent Cytomegalovirus Transcription Drive CD8 T-Cell "Memory Inflation" and Avoid Immune Evasion.

Authors:  Marion Griessl; Angelique Renzaho; Kirsten Freitag; Christof K Seckert; Matthias J Reddehase; Niels A W Lemmermann
Journal:  Front Immunol       Date:  2021-04-22       Impact factor: 7.561

Review 8.  Reverse genetics modification of cytomegalovirus antigenicity and immunogenicity by CD8 T-cell epitope deletion and insertion.

Authors:  Niels A W Lemmermann; Kai A Kropp; Christof K Seckert; Natascha K A Grzimek; Matthias J Reddehase
Journal:  J Biomed Biotechnol       Date:  2010-12-26

Review 9.  Viral enhancer mimicry of host innate-immune promoters.

Authors:  Kai A Kropp; Ana Angulo; Peter Ghazal
Journal:  PLoS Pathog       Date:  2014-02-06       Impact factor: 6.823

10.  A new reporter mouse cytomegalovirus reveals maintained immediate-early gene expression but poor virus replication in cycling liver sinusoidal endothelial cells.

Authors:  Franziska Dag; Adrien Weingärtner; Milada Butueva; Ianina Conte; Julia Holzki; Tobias May; Barbara Adler; Dagmar Wirth; Luka Cicin-Sain
Journal:  Virol J       Date:  2013-06-17       Impact factor: 4.099
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