Literature DB >> 19551360

Application of fluorescence polarization in HTS assays.

Xinyi Huang1, Ann Aulabaugh.   

Abstract

Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

Mesh：

Year:  2009        PMID： 19551360     DOI： 10.1007/978-1-60327-258-2_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Structural mechanism for HIV-1 TAR loop recognition by Tat and the super elongation complex.

Authors:  Ursula Schulze-Gahmen; James H Hurley
Journal:  Proc Natl Acad Sci U S A       Date:  2018-12-04       Impact factor: 11.205

2.  A small-molecule mimic of a peptide docking motif inhibits the protein kinase PDK1.

Authors:  T Justin Rettenmaier; Jack D Sadowsky; Nathan D Thomsen; Steven C Chen; Allison K Doak; Michelle R Arkin; James A Wells
Journal:  Proc Natl Acad Sci U S A       Date:  2014-12-17       Impact factor: 11.205

3.  Fluorescence polarization assays in high-throughput screening and drug discovery: a review.

Authors:  Matthew D Hall; Adam Yasgar; Tyler Peryea; John C Braisted; Ajit Jadhav; Anton Simeonov; Nathan P Coussens
Journal:  Methods Appl Fluoresc       Date:  2016-04-28       Impact factor: 3.009

4.  Comparison of Förster-resonance-energy-transfer acceptors for tryptophan and tyrosine residues in native proteins as donors.

Authors:  Yi zhang; Xiaolan Yang; Lin Liu; Xuexia Huang; Zuexia Huang; Jun Pu; Gaobo Long; Ling Zhang; Dong Liu; Bangtian Xu; Juan Liao; Fei Liao
Journal:  J Fluoresc       Date:  2012-09-22       Impact factor: 2.217

5.  Identification of influenza endonuclease inhibitors using a novel fluorescence polarization assay.

Authors:  Brandi M Baughman; P Jake Slavish; Rebecca M DuBois; Vincent A Boyd; Stephen W White; Thomas R Webb
Journal:  ACS Chem Biol       Date:  2012-01-19       Impact factor: 5.100

6.  Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform.

Authors:  Tobias Wagner; Holger Greschik; Teresa Burgahn; Karin Schmidtkunz; Anne-Kathrin Schott; Joel McMillan; Lina Baranauskienė; Yan Xiong; Oleg Fedorov; Jian Jin; Udo Oppermann; Daumantas Matulis; Roland Schüle; Manfred Jung
Journal:  Nucleic Acids Res       Date:  2016-02-17       Impact factor: 16.971

7.  In vitro screening and structural characterization of inhibitors of the S100B-p53 interaction.

Authors:  Paul T Wilder; Thomas H Charpentier; Melissa A Liriano; Kira Gianni; Kristen M Varney; Edwin Pozharski; Andrew Coop; Eric A Toth; Alex D Mackerell; David J Weber
Journal:  Int J High Throughput Screen       Date:  2010-07-07

8.  A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents.

Authors:  Paul P Geurink; Farid El Oualid; Anika Jonker; Dharjath S Hameed; Huib Ovaa
Journal:  Chembiochem       Date:  2011-12-23       Impact factor: 3.164

9.  Tanshinones: First-in-Class Inhibitors of the Biogenesis of the Type 3 Secretion System Needle of Pseudomonas aeruginosa for Antibiotic Therapy.

Authors:  Chao Feng; Yinong Huang; Wangxiao He; Xiyao Cheng; Huili Liu; Yongqi Huang; Bohan Ma; Wei Zhang; Chongbing Liao; Weihui Wu; Yongping Shao; Dan Xu; Zhengding Su; Wuyuan Lu
Journal:  ACS Cent Sci       Date:  2019-06-26       Impact factor: 14.553

10.  Orthogonal ring-closing alkyne and olefin metathesis for the synthesis of small GTPase-targeting bicyclic peptides.

Authors:  Philipp M Cromm; Sebastian Schaubach; Jochen Spiegel; Alois Fürstner; Tom N Grossmann; Herbert Waldmann
Journal:  Nat Commun       Date:  2016-04-14       Impact factor: 14.919
  10 in total

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