Literature DB >> 19550692

Label-free optical imaging of mitochondria in live cells.

David Lasne, Gerhard A Blab, Francesca De Giorgi, François Ichas, Brahim Lounis, Laurent Cognet.   

Abstract

The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences.

Entities:  

Year:  2007        PMID： 19550692     DOI： 10.1364/oe.15.014184

Source DB:  PubMed          Journal:  Opt Express        ISSN： 1094-4087            Impact factor:   3.894


  15 in total

1.  Simultaneous immersion Mirau interferometry.

Authors:  Oleksandra V Lyulko; Gerhard Randers-Pehrson; David J Brenner
Journal:  Rev Sci Instrum       Date:  2013-05       Impact factor: 1.523

2.  Photothermal imaging of skeletal muscle mitochondria.

Authors:  Toru Tomimatsu; Jun Miyazaki; Yutaka Kano; Takayoshi Kobayashi
Journal:  Biomed Opt Express       Date:  2017-05-15       Impact factor: 3.732

3.  Label-free photoacoustic microscopy of cytochromes.

Authors:  Chi Zhang; Yu Shrike Zhang; Da-Kang Yao; Younan Xia; Lihong V Wang
Journal:  J Biomed Opt       Date:  2013-02       Impact factor: 3.170

4.  Mid-infrared photothermal heterodyne spectroscopy in a liquid crystal using a quantum cascade laser.

Authors:  Alket Mërtiri; Thomas Jeys; Vladimir Liberman; M K Hong; Jerome Mertz; Hatice Altug; Shyamsunder Erramilli
Journal:  Appl Phys Lett       Date:  2012-07-23       Impact factor: 3.791

5.  Ultrasensitive label-free photothermal imaging, spectral identification, and quantification of cytochrome c in mitochondria, live cells, and solutions.

Authors:  Anton V Brusnichkin; Dmitry A Nedosekin; Ekaterina I Galanzha; Yuri A Vladimirov; Elena F Shevtsova; Mikhail A Proskurnin; Vladimir P Zharov
Journal:  J Biophotonics       Date:  2010-12       Impact factor: 3.207

6.  Photothermal multispectral image cytometry for quantitative histology of nanoparticles and micrometastasis in intact, stained and selectively burned tissues.

Authors:  Dmitry A Nedosekin; Evgeny V Shashkov; Ekaterina I Galanzha; Leah Hennings; Vladimir P Zharov
Journal:  Cytometry A       Date:  2010-11       Impact factor: 4.355

7.  Photothermal confocal spectromicroscopy of multiple cellular chromophores and fluorophores.

Authors:  Dmitry A Nedosekin; Ekaterina I Galanzha; Srinivas Ayyadevara; Robert J Shmookler Reis; Vladimir P Zharov
Journal:  Biophys J       Date:  2012-02-07       Impact factor: 4.033

8.  Photothermal Microscopy: Imaging the Optical Absorption of Single Nanoparticles and Single Molecules.

Authors:  Subhasis Adhikari; Patrick Spaeth; Ashish Kar; Martin Dieter Baaske; Saumyakanti Khatua; Michel Orrit
Journal:  ACS Nano       Date:  2020-11-20       Impact factor: 15.881

9.  Label-free dynamic imaging of mitochondria and lysosomes within living cells via simultaneous dual-pump photothermal microscopy.

Authors:  Jun Miyazaki; Yasunobu Toumon
Journal:  Biomed Opt Express       Date:  2019-10-23       Impact factor: 3.732

10.  Transport of fibroblast growth factor 2 in the pericellular matrix is controlled by the spatial distribution of its binding sites in heparan sulfate.

Authors:  Laurence Duchesne; Vivien Octeau; Rachel N Bearon; Alison Beckett; Ian A Prior; Brahim Lounis; David G Fernig
Journal:  PLoS Biol       Date:  2012-07-17       Impact factor: 8.029
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