AIMS: Isoprostanes may serve as sensitive and specific markers of in vivo oxidative stress intensity. We wanted to determine, whether or not isoprostane concentration may be considered as a risk marker of premature rupture of fetal membranes (PROM). METHODS: On the basis of the presence of PROM and gestational maturity, a total of 128 patients were divided into: (1) preterm PROM (pPROM) group; (2) PROM at term group; (3) control preterm (C1) group and (4) control at term (C2) group. The concentrations of 8-iPF(2alpha)-III were determined using the enzyme-linked immunosorbent assay method. RESULTS: The mean free isoprostane concentrations, examined in amniotic fluid and maternal plasma in the PROM at term patients were significantly higher than in C2 individuals (p < 0.01). The mean concentrations of free 8-iPF(2alpha)-III measured in blood plasma from women in the C1 group were significantly lower than in patients from the pPROM, PROM at term and C2 groups (p < 0.001, p < 0.00001 and p < 0.00001, respectively). CONCLUSION: The measurement of free isoprostane concentration in maternal plasma and amniotic fluid may be considered as a laboratory marker of a PROM-risk pregnancy.
AIMS: Isoprostanes may serve as sensitive and specific markers of in vivo oxidative stress intensity. We wanted to determine, whether or not isoprostane concentration may be considered as a risk marker of premature rupture of fetal membranes (PROM). METHODS: On the basis of the presence of PROM and gestational maturity, a total of 128 patients were divided into: (1) preterm PROM (pPROM) group; (2) PROM at term group; (3) control preterm (C1) group and (4) control at term (C2) group. The concentrations of 8-iPF(2alpha)-III were determined using the enzyme-linked immunosorbent assay method. RESULTS: The mean free isoprostane concentrations, examined in amniotic fluid and maternal plasma in the PROM at term patients were significantly higher than in C2 individuals (p < 0.01). The mean concentrations of free 8-iPF(2alpha)-III measured in blood plasma from women in the C1 group were significantly lower than in patients from the pPROM, PROM at term and C2 groups (p < 0.001, p < 0.00001 and p < 0.00001, respectively). CONCLUSION: The measurement of free isoprostane concentration in maternal plasma and amniotic fluid may be considered as a laboratory marker of a PROM-risk pregnancy.
Authors: Kelly K Ferguson; Thomas F McElrath; Yin-Hsiu Chen; Rita Loch-Caruso; Bhramar Mukherjee; John D Meeker Journal: Am J Obstet Gynecol Date: 2014-08-08 Impact factor: 8.661
Authors: Emma M Rosen; Thomas J van 't Erve; Jonathan Boss; Sheela Sathyanarayana; Emily S Barrett; Ruby H N Nguyen; Nicole R Bush; Ginger L Milne; Thomas F McElrath; Shanna H Swan; Kelly K Ferguson Journal: Free Radic Biol Med Date: 2018-11-14 Impact factor: 7.376
Authors: Linda Englund-Ögge; Anne Lise Brantsæter; Verena Sengpiel; Margareta Haugen; Bryndis Eva Birgisdottir; Ronny Myhre; Helle Margrete Meltzer; Bo Jacobsson Journal: BMJ Date: 2014-03-04
Authors: Rafał Rzepka; Barbara Dołegowska; Aleksandra Rajewska; Sebastian Kwiatkowski; Daria Sałata; Marta Budkowska; Leszek Domański; Wioletta Mikołajek-Bedner; Andrzej Torbé Journal: Biomed Res Int Date: 2015-08-27 Impact factor: 3.411