PURPOSE: We developed a mouse model that enables non-invasive assessment of changes in beta cell mass. PROCEDURES: We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased beta cell mass and after islet transplantation. RESULTS: Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in beta cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in beta cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year. CONCLUSIONS: Bioluminescence in the MIP-Luc-VU mouse model is proportional to beta cell mass in the setting of increased and decreased beta cell mass and after transplantation.
PURPOSE: We developed a mouse model that enables non-invasive assessment of changes in beta cell mass. PROCEDURES: We generated a transgenicmouse expressing luciferase under control of the mouseinsulin I promoter [mouseinsulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased beta cell mass and after islet transplantation. RESULTS:Streptozotocin-induced, diabetic MIP-Luc-VUmice had a progressive decline in bioluminescence that correlated with a decrease in beta cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in beta cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year. CONCLUSIONS: Bioluminescence in the MIP-Luc-VU mouse model is proportional to beta cell mass in the setting of increased and decreased beta cell mass and after transplantation.
Authors: C H Contag; S D Spilman; P R Contag; M Oshiro; B Eames; P Dennery; D K Stevenson; D A Benaron Journal: Photochem Photobiol Date: 1997-10 Impact factor: 3.421
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