Min-Jung Lee1, Sook-Kyung Lee, Kyung-Eun Lee, Hee-Yun Kang, Han-Sung Jung, Jung-Wook Kim. 1. Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Republic of Korea.
Abstract
AIM: Recently a novel gene, FAM83H, was identified by a genetic linkage study in the hypocalcified form of the amelogenesis imperfecta family with an autosomal dominant hereditary pattern. Little is known about this novel gene, and so we investigated the expression pattern of Fam83h in murine tooth development using serial sectional in situ hybridisation. METHODS AND MATERIALS: Using mandibles of ICR mouse at specific developmental stages, in situ hybridisation was performed by DIG-labeled RNA probe. RESULTS: Faint expression was detected in limited cells at embryonic day 14 (E14) in the molar. At the bell stage, E16, Fam83h was localised in the outer and inner enamel epithelium, as well as dental papilla. Fam83h expression begins on E15 in the developing incisor. At E18, Fam83h was expressed in the inner enamel epithelium of the apical bud, ameloblasts and odontoblasts. The expression was stronger in the presecretory stages than the secretory stages. CONCLUSION: Fam83h was detected in the ameloblasts from the presecretory to the secretory stage, and also the odontoblasts layer and surrounding alveolar bone.
AIM: Recently a novel gene, FAM83H, was identified by a genetic linkage study in the hypocalcified form of the amelogenesis imperfecta family with an autosomal dominant hereditary pattern. Little is known about this novel gene, and so we investigated the expression pattern of Fam83h in murine tooth development using serial sectional in situ hybridisation. METHODS AND MATERIALS: Using mandibles of ICR mouse at specific developmental stages, in situ hybridisation was performed by DIG-labeled RNA probe. RESULTS: Faint expression was detected in limited cells at embryonic day 14 (E14) in the molar. At the bell stage, E16, Fam83h was localised in the outer and inner enamel epithelium, as well as dental papilla. Fam83h expression begins on E15 in the developing incisor. At E18, Fam83h was expressed in the inner enamel epithelium of the apical bud, ameloblasts and odontoblasts. The expression was stronger in the presecretory stages than the secretory stages. CONCLUSION:Fam83h was detected in the ameloblasts from the presecretory to the secretory stage, and also the odontoblasts layer and surrounding alveolar bone.
Authors: S-K Lee; F Seymen; K-E Lee; H-Y Kang; M Yildirim; E Bahar Tuna; K Gencay; Y-H Hwang; K H Nam; R J De La Garza; J C-C Hu; J P Simmer; J-W Kim Journal: J Dent Res Date: 2010-10-11 Impact factor: 6.116
Authors: Hui-Chen Chan; Ninna M R P Estrella; Rachel N Milkovich; Jung-Wook Kim; James P Simmer; Jan C-C Hu Journal: Eur J Oral Sci Date: 2011-12 Impact factor: 2.612
Authors: Walid El-Sayed; David A Parry; Roger C Shore; Mushtaq Ahmed; Hussain Jafri; Yasmin Rashid; Suhaila Al-Bahlani; Sharifa Al Harasi; Jennifer Kirkham; Chris F Inglehearn; Alan J Mighell Journal: Am J Hum Genet Date: 2009-10-22 Impact factor: 11.025