Simple two-layer reflectance model for biological tissue applications.

A two-layer tissue diffuse reflectance model is described. The model is based on a simple one-layer model that we have recently developed and successfully applied to the analysis of in vivo skin reflectance. The model, which is specifically designed for use with a fiber optic probe, has as its main features simplicity and ease of application, and it is capable of estimating the thickness and the absorption coefficient of a superficial absorbing and scattering layer. Both of these parameters are of great interest for the noninvasive study of epithelial biological tissues. The validity range and accuracy of the model are tested on tissue phantoms in both the forward and inverse modes of application.