| Literature DB >> 19540540 |
Dan Staerk1, Julie R Kesting, Majid Sairafianpour, Matthias Witt, Javad Asili, Seyed A Emami, Jerzy W Jaroszewski.
Abstract
Direct hyphenation of analytical-scale high-performance liquid chromatography, photo-diode array detection, mass spectrometry, solid-phase extraction and nuclear magnetic resonance spectroscopy (HPLC-PDA-MS-SPE-NMR) has been used for accelerated dereplication of crude extract of Haplophyllum acutifolium (syn. Haplophyllum perforatum). This technique allowed fast on-line identification of six quinolinone alkaloids, named haplacutine A-F, as well as of acutine, haplamine, eudesmine, and 2-nonylquinolin-4(1H)-one. Acutine and haplacutine E, isolated by preparative-scale HPLC, showed moderate antiplasmodial activity with IC(50) values of 2.17+/-0.22 microM and 3.79+/-0.24 microM, respectively (chloroquine-sensitive Plasmodium falciparum 3D7 strain).Entities:
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Year: 2009 PMID： 19540540 DOI： 10.1016/j.phytochem.2009.05.004
Source DB: PubMed Journal: Phytochemistry ISSN： 0031-9422 Impact factor: 4.072