| Literature DB >> 1954015 |
R J Zagursky1, P S Conway, M A Kashdan.
Abstract
The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like 32P, yet they maintain band resolution similar to 35S. Handling of [alpha-33P]dATP is straightforward because no special lead or Plexiglas shielding is necessary.Entities:
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Year: 1991 PMID： 1954015
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993