Literature DB >> 1954010

Chemiluminescent substrates increase sensitivity of antigen detection in western blots.

G S Sandhu1, B W Eckloff, B C Kline.   

Abstract

Replacement of colorimetric substrates in Western blots by chemiluminescent reagents enhances the sensitivity of detection by more than an order of magnitude. Protein levels beyond the detection limit of colorimetric substrates can be consistently detected without modifying pre-existing laboratory protocols.

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Year:  1991        PMID: 1954010

Source DB:  PubMed          Journal:  Biotechniques        ISSN: 0736-6205            Impact factor:   1.993


  9 in total

1.  HIV-1 LTR C/EBP binding site sequence configurations preferentially encountered in brain lead to enhanced C/EBP factor binding and increased LTR-specific activity.

Authors:  H L Ross; S Gartner; J C McArthur; J R Corboy; J J McAllister; S Millhouse; B Wigdahl
Journal:  J Neurovirol       Date:  2001-06       Impact factor: 2.643

2.  The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.

Authors:  S Dou; X Zeng; P Cortes; H Erdjument-Bromage; P Tempst; T Honjo; L D Vales
Journal:  Mol Cell Biol       Date:  1994-05       Impact factor: 4.272

3.  Structural and functional evolution of human immunodeficiency virus type 1 long terminal repeat CCAAT/enhancer binding protein sites and their use as molecular markers for central nervous system disease progression.

Authors:  Tricia H Hogan; Devin L Stauff; Fred C Krebs; Suzanne Gartner; Shane J Quiterio; Brian Wigdahl
Journal:  J Neurovirol       Date:  2003-02       Impact factor: 2.643

4.  Hemolytically active (acylated) alpha-hemolysin elicits interleukin-1beta (IL-1beta) but augments the lethality of Escherichia coli by an IL-1- and tumor necrosis factor-independent mechanism.

Authors:  T G Gleason; C W Houlgrave; A K May; T D Crabtree; R G Sawyer; W Denham; J G Norman; T L Pruett
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

5.  Characterization of a Novel Strain of Bacillus thuringiensis.

Authors:  J E Lopez-Meza; J E Ibarra
Journal:  Appl Environ Microbiol       Date:  1996-04       Impact factor: 4.792

6.  Interaction between CCAAT/enhancer binding protein and cyclic AMP response element binding protein 1 regulates human immunodeficiency virus type 1 transcription in cells of the monocyte/macrophage lineage.

Authors:  H L Ross; M R Nonnemacher; T H Hogan; S J Quiterio; A Henderson; J J McAllister; F C Krebs; B Wigdahl
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

7.  Mass Spectrometry versus Conventional Techniques of Protein Detection: Zika Virus NS3 Protease Activity towards Cellular Proteins.

Authors:  Agnieszka Dabrowska; Aleksandra Milewska; Joanna Ner-Kluza; Piotr Suder; Krzysztof Pyrc
Journal:  Molecules       Date:  2021-06-18       Impact factor: 4.411

8.  Constitutive expression of interferon gamma-inducible protein 10 in lymphoid organs and inducible expression in T cells and thymocytes.

Authors:  C R Gattass; L B King; A D Luster; J D Ashwell
Journal:  J Exp Med       Date:  1994-04-01       Impact factor: 14.307

9.  Human gamma-tubulin functions in fission yeast.

Authors:  T Horio; B R Oakley
Journal:  J Cell Biol       Date:  1994-09       Impact factor: 10.539

  9 in total

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