| Literature DB >> 19539774 |
Kwang Sik Lee1, Bo Yeon Kim, Young Moo Choo, Hyung Joo Yoon, Pil Don Kang, Soo Dong Woo, Hung Dae Sohn, Jong Yul Roh, Zhong Zheng Gui, Yeon Ho Je, Byung Rae Jin.
Abstract
Proteolytic enzymes are involved in insect molting and metamorphosis, and play a vital role in the programmed cell death of obsolete organs. Here we show the expression profile of cathepsin B in the fat body of the silkworm Bombyx mori during development. We also compare the expression profiles of B. mori cathepsins B (BmCatB) and D (BmCatD) during normal development and after RNA interference (RNAi)-mediated inhibition. BmCatB is induced by 20-OH-ecdysone, and is expressed in the fat body of B. mori during molting and the larval-pupal and pupal-adult transformations, where its expression leads to programmed cell death. In particular, BmCatB is highly expressed in the fat body of B. mori during the larval-pupal transformation, and BmCatB RNAi treatment resulted in an arrest of the larval-pupal transformation. RNAi-mediated BmCatB knockdown sustained the expression of BmCatD during the larval-pupal transformation. On the other hand, when BmCatD was inhibited via RNAi, the expression of BmCatB was upregulated. Based on these results, we conclude that BmCatB is involved in the programmed cell death of the fat body during B. mori metamorphosis, and that BmCatB and BmCatD contribute to B. mori metamorphosis.Entities:
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Year: 2009 PMID： 19539774 DOI： 10.1016/j.cbpb.2009.06.002
Source DB: PubMed Journal: Comp Biochem Physiol B Biochem Mol Biol ISSN： 1096-4959 Impact factor: 2.231