BACKGROUND: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic. OBJECTIVES: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1. STUDY DESIGN: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods. RESULTS: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID(50)) log(10)3.3-4.7 was comparable with that of seasonal influenza (TCID(50) log(10)4.0-4.5). CONCLUSION: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.
BACKGROUND: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic. OBJECTIVES: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1. STUDY DESIGN: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods. RESULTS: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID(50)) log(10)3.3-4.7 was comparable with that of seasonal influenza (TCID(50) log(10)4.0-4.5). CONCLUSION: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenzaH1N1.
Authors: Mercedes Pérez-Ruiz; José-María Navarro-Marí; María-Fé Bautista-Marín; Irene Pedrosa-Corral; Sara Sanbonmatsu-Gámez; Ana G Camacho; José Rojas; Jorge Ruiz-Ortiz; Javier Rodríguez-Granger; José Antonio Carrillo Journal: J Clin Microbiol Date: 2010-03-10 Impact factor: 5.948
Authors: F Stripeli; Z Sakkou; N Papadopoulos; V Georgiou; P Gratsia; I Christodoulou; M Tsolia Journal: Eur J Clin Microbiol Infect Dis Date: 2010-03-27 Impact factor: 3.267
Authors: Janice K Louie; Hugo Guevara; Erica Boston; Melissa Dahlke; Maria Nevarez; Tong Kong; Robert Schechter; Carol A Glaser; David P Schnurr Journal: Emerg Infect Dis Date: 2010-05 Impact factor: 6.883
Authors: Ludhmila Abrahao Hajjar; Denise Schout; Filomena Regina Barbosa Gomes Galas; David Everson Uip; Anna Sara Shafferman Levin; Helio Hehl Caiaffa Filho; Pedro Takanori Sakane; Carlos Alberto Suslik; Jose Manoel de Camargo Teixeira; Eloisa Bonfa; Antonio Alci Barone; Milton de Arruda Martins; Marcos Boulos; Jose Otavio Costa Auler Journal: Clinics (Sao Paulo) Date: 2009 Impact factor: 2.365
Authors: Adrian K Ong; Mark I Chen; Li Lin; Adriana S Tan; Ni Win Nwe; Timothy Barkham; Seow Yian Tay; Yee Sin Leo Journal: PLoS One Date: 2009-12-29 Impact factor: 3.240