Literature DB >> 195376

Canine urinary bladder epithelial cells: preparation for cell culture by enzyme dispersion.

R A Bonar, C F Reich, Y Sharief.   

Abstract

In a qualitative and quantitative study of enzymic dispersion of cells from the mucosal layer stripped from canine urinary bladder, trypsin was found to be equal or superior to the other enzymes tested for dispersal of urothelial cells specifically. Collagenase or collagenase plus trypsin served to disperse the whole tissue. A procedure for recovering the urothelial cells as a single-cell suspension and establishing them in culture is presented. The morphology, culture behaviour, and chromosome complement of these cells is described.

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Year:  1977        PMID： 195376     DOI： 10.1007/bf00256846

Source DB:  PubMed          Journal:  Urol Res        ISSN： 0300-5623


  17 in total

1.  In vitro culture of epithelial cells derived from urogenital tissues.

Authors:  K R Stone; D F Paulson; R A Bonar; C F Reich
Journal:  Urol Res       Date:  1975

2.  Cyto-dynamic properties of urinary neoplasms. V. Cultivation in vitro of transitional cell epithelium.

Authors:  R G BUNGE
Journal:  J Urol       Date:  1955-01       Impact factor: 7.450

3.  Cyto-dynamic properties of urinary neoplasms. I. Cultivation in vitro of transitional cell carcinoma of the ureter.

Authors:  R G BUNGE; R J STEIN
Journal:  J Urol       Date:  1950-11       Impact factor: 7.450

4.  Immunological and cultural characteristics of urothelium.

Authors:  J J Daly; G R Prout; C A Ahl
Journal:  J Urol       Date:  1972-04       Impact factor: 7.450

5.  A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium.

Authors:  J Leighton; L W Estes; S Mansukhani; Z Brada
Journal:  Cancer       Date:  1970-11       Impact factor: 6.860

6.  The immediate response of bladder epithelium to injury by chemical carcinogens.

Authors:  D B Clayson; E H Cooper
Journal:  Br J Urol       Date:  1969-12

7.  Cellular and humoral immune responses to human urinary bladder carcinomas.

Authors:  J Bubeník; P Perlmann; K Helmstein; G Moberger
Journal:  Int J Cancer       Date:  1970-05-15       Impact factor: 7.396

Review 8.  Cancer of the urinary tract.

Authors:  D B Clayson; E H Cooper
Journal:  Adv Cancer Res       Date:  1970       Impact factor: 6.242

9.  Leukocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KCl.

Authors:  D A Hungerford
Journal:  Stain Technol       Date:  1965-11

10.  Cell kinetics of mouse urinary bladder epithelium. I. Circadian and age variations in cell proliferation and nuclear DNA content.

Authors:  T Farsund
Journal:  Virchows Arch B Cell Pathol       Date:  1975
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  4 in total

1.  Growth and characterization of normal human urothelium in vitro.

Authors:  C A Reznikoff; M D Johnson; D H Norback; G T Bryan
Journal:  In Vitro       Date:  1983-04

2.  Long-term culture of porcine bladder epithelial cells.

Authors:  Ursula K Ehmann; Martha K Terris
Journal:  In Vitro Cell Dev Biol Anim       Date:  2002-03       Impact factor: 2.416

3.  Polyploidy in mammalian urothelial cells.

Authors:  Y Sharief; C F Reich; R A Bonar
Journal:  Urol Res       Date:  1980

4.  The growth and differentiation of transitional epithelium in vitro.

Authors:  F J Chlapowski; L Haynes
Journal:  J Cell Biol       Date:  1979-12       Impact factor: 10.539
  4 in total

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