Literature DB >> 19532862

Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme.

Aélig Robin1, Gareth A Roberts, Johannes Kisch, Federico Sabbadin, Gideon Grogan, Neil Bruce, Nicholas J Turner, Sabine L Flitsch.   

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19532862     DOI： 10.1039/b901716j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  16 in total

1.  Discovery of potent parthenolide-based antileukemic agents enabled by late-stage P450-mediated C-H functionalization.

Authors:  Joshua N Kolev; Kristen M O'Dwyer; Craig T Jordan; Rudi Fasan
Journal:  ACS Chem Biol       Date:  2013-11-08       Impact factor: 5.100

Review 2.  Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications.

Authors:  Zhong Li; Yuanyuan Jiang; F Peter Guengerich; Li Ma; Shengying Li; Wei Zhang
Journal:  J Biol Chem       Date:  2019-12-06       Impact factor: 5.157

Review 3.  Cytochrome P450: taming a wild type enzyme.

Authors:  Sang Taek Jung; Ryan Lauchli; Frances H Arnold
Journal:  Curr Opin Biotechnol       Date:  2011-03-14       Impact factor: 9.740

4.  Biochemical and Structural Characterization of MycCI, a Versatile P450 Biocatalyst from the Mycinamicin Biosynthetic Pathway.

Authors:  Matthew D DeMars; Fang Sheng; Sung Ryeol Park; Andrew N Lowell; Larissa M Podust; David H Sherman
Journal:  ACS Chem Biol       Date:  2016-08-05       Impact factor: 5.100

5.  Enhancing the efficiency and regioselectivity of P450 oxidation catalysts by unnatural amino acid mutagenesis.

Authors:  Joshua N Kolev; Jacqueline M Zaengle; Rajesh Ravikumar; Rudi Fasan
Journal:  Chembiochem       Date:  2014-04-01       Impact factor: 3.164

6.  Partial fusion of a cytochrome P450 system by carboxy-terminal attachment of putidaredoxin reductase to P450cam (CYP101A1).

Authors:  Eachan O Johnson; Luet-Lok Wong
Journal:  Catal Sci Technol       Date:  2016-09-01       Impact factor: 6.119

7.  Biocatalytic synthesis of flavones and hydroxyl-small molecules by recombinant Escherichia coli cells expressing the cyanobacterial CYP110E1 gene.

Authors:  Takuya Makino; Toshihiko Otomatsu; Kazutoshi Shindo; Emi Kitamura; Gerhard Sandmann; Hisashi Harada; Norihiko Misawa
Journal:  Microb Cell Fact       Date:  2012-07-18       Impact factor: 5.328

8.  Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope.

Authors:  Aélig Robin; Valentin Köhler; Alison Jones; Afruja Ali; Paul P Kelly; Elaine O'Reilly; Nicholas J Turner; Sabine L Flitsch
Journal:  Beilstein J Org Chem       Date:  2011-11-02       Impact factor: 2.883

9.  Controlled oxidation of remote sp3 C-H bonds in artemisinin via P450 catalysts with fine-tuned regio- and stereoselectivity.

Authors:  Kaidong Zhang; Brian M Shafer; Matthew D Demars; Harry A Stern; Rudi Fasan
Journal:  J Am Chem Soc       Date:  2012-11-02       Impact factor: 15.419

10.  New reactions and products resulting from alternative interactions between the P450 enzyme and redox partners.

Authors:  Wei Zhang; Yi Liu; Jinyong Yan; Shaona Cao; Fali Bai; Ying Yang; Shaohua Huang; Lishan Yao; Yojiro Anzai; Fumio Kato; Larissa M Podust; David H Sherman; Shengying Li
Journal:  J Am Chem Soc       Date:  2014-02-25       Impact factor: 15.419
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.