Literature DB >> 19532790

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy.

Kai Hassler, Per Rigler, Hans Blom, Rudolf Rigler, Jerker Widengren, Theo Lasser.   

Abstract

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.

Entities:  

Year:  2007        PMID： 19532790     DOI： 10.1364/oe.15.005366

Source DB:  PubMed          Journal:  Opt Express        ISSN： 1094-4087            Impact factor:   3.894


  10 in total

1.  Interrogating the activities of conformational deformed enzyme by single-molecule fluorescence-magnetic tweezers microscopy.

Authors:  Qing Guo; Yufan He; H Peter Lu
Journal:  Proc Natl Acad Sci U S A       Date:  2015-10-28       Impact factor: 11.205

2.  Vesicle diffusion close to a membrane: intermembrane interactions measured with fluorescence correlation spectroscopy.

Authors:  Minjoung Kyoung; Erin D Sheets
Journal:  Biophys J       Date:  2008-10-17       Impact factor: 4.033

3.  Probing conformational dynamics of an enzymatic active site by an in situ single fluorogenic probe under piconewton force manipulation.

Authors:  Nibedita Pal; Meiling Wu; H Peter Lu
Journal:  Proc Natl Acad Sci U S A       Date:  2016-12-08       Impact factor: 11.205

4.  Quantifying the flux as the driving force for nonequilibrium dynamics and thermodynamics in non-Michaelis-Menten enzyme kinetics.

Authors:  Qiong Liu; Jin Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2019-12-26       Impact factor: 11.205

5.  Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations.

Authors:  Nancy L Thompson; Punya Navaratnarajah; Xiang Wang
Journal:  J Phys Chem B       Date:  2010-12-17       Impact factor: 2.991

6.  Enantioselective Synthesis of Dilignol Model Compounds and Their Stereodiscrimination Study with a Dye-Decolorizing Peroxidase.

Authors:  Gaochao Huang; Ruben Shrestha; Kaimin Jia; Brian V Geisbrecht; Ping Li
Journal:  Org Lett       Date:  2017-03-22       Impact factor: 6.005

7.  Electrostatic interactions of fluorescent molecules with dielectric interfaces studied by total internal reflection fluorescence correlation spectroscopy.

Authors:  Hans Blom; Kai Hassler; Andriy Chmyrov; Jerker Widengren
Journal:  Int J Mol Sci       Date:  2010-01-28       Impact factor: 5.923

8.  Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.

Authors:  Nancy L Thompson; Xiang Wang; Punya Navaratnarajah
Journal:  J Struct Biol       Date:  2009-03-06       Impact factor: 2.867

9.  Single-molecule enzymatic conformational dynamics: spilling out the product molecules.

Authors:  Desheng Zheng; H Peter Lu
Journal:  J Phys Chem B       Date:  2014-07-28       Impact factor: 2.991

10.  Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy.

Authors:  Jonas Mücksch; Philipp Blumhardt; Maximilian T Strauss; Eugene P Petrov; Ralf Jungmann; Petra Schwille
Journal:  Nano Lett       Date:  2018-04-18       Impact factor: 11.189
  10 in total

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