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Literature DB >> 19531802

Using a microfluidic device for high-content analysis of cell signaling.

Raymond Cheong¹, Chiaochun Joanne Wang, Andre Levchenko.

Abstract

Quantitative analysis and understanding of signaling networks require measurements of the location and activities of key proteins over time, at the level of single cells, in response to various perturbations. Microfluidic devices enable such analyses to be conducted in a high-throughput and in a highly controlled manner. We describe in detail how to design and use a microfluidic device to perform such information-rich experiments.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Year: 2009 PMID： 19531802 PMCID： PMC3918417 DOI： 10.1126/scisignal.275pl2

Source DB: PubMed Journal: Sci Signal ISSN： 1945-0877 Impact factor: 8.192

21 in total

1. Monolithic microfabricated valves and pumps by multilayer soft lithography.

Authors: M A Unger; H P Chou; T Thorsen; A Scherer; S R Quake
Journal: Science Date: 2000-04-07 Impact factor: 47.728

2. Microfluidic large-scale integration.

Authors: Todd Thorsen; Sebastian J Maerkl; Stephen R Quake
Journal: Science Date: 2002-09-26 Impact factor: 47.728

3. Causal protein-signaling networks derived from multiparameter single-cell data.

Authors: Karen Sachs; Omar Perez; Dana Pe'er; Douglas A Lauffenburger; Garry P Nolan
Journal: Science Date: 2005-04-22 Impact factor: 47.728

4. Comment on "Oscillations in NF-kappaB signaling control the dynamics of gene expression".

Authors: Derren Barken; Chiaochun Joanne Wang; Jeff Kearns; Raymond Cheong; Alexander Hoffmann; Andre Levchenko
Journal: Science Date: 2005-04-01 Impact factor: 47.728

5. A systems model of signaling identifies a molecular basis set for cytokine-induced apoptosis.

Authors: Kevin A Janes; John G Albeck; Suzanne Gaudet; Peter K Sorger; Douglas A Lauffenburger; Michael B Yaffe
Journal: Science Date: 2005-12-09 Impact factor: 47.728

6. A plate reader-compatible microchannel array for cell biology assays.

Authors: Hongmei Yu; Caroline M Alexander; David J Beebe
Journal: Lab Chip Date: 2007-01-02 Impact factor: 6.799

7. The use of a charge-coupled device for quantitative optical microscopy of biological structures.

Authors: Y Hiraoka; J W Sedat; D A Agard
Journal: Science Date: 1987-10-02 Impact factor: 47.728

8. The response of human epithelial cells to TNF involves an inducible autocrine cascade.

Authors: Kevin A Janes; Suzanne Gaudet; John G Albeck; Ulrik B Nielsen; Douglas A Lauffenburger; Peter K Sorger
Journal: Cell Date: 2006-03-24 Impact factor: 41.582

9. Single cell profiling of potentiated phospho-protein networks in cancer cells.

Authors: Jonathan M Irish; Randi Hovland; Peter O Krutzik; Omar D Perez; Øystein Bruserud; Bjørn T Gjertsen; Garry P Nolan
Journal: Cell Date: 2004-07-23 Impact factor: 41.582

10. Enhanced sensitivity to IGF-II signaling links loss of imprinting of IGF2 to increased cell proliferation and tumor risk.

Authors: Atsushi Kaneda; Chiaochun J Wang; Raymond Cheong; Winston Timp; Patrick Onyango; Bo Wen; Christine A Iacobuzio-Donahue; Rolf Ohlsson; Rita Andraos; Mark A Pearson; Alexei A Sharov; Dan L Longo; Minoru S H Ko; Andre Levchenko; Andrew P Feinberg
Journal: Proc Natl Acad Sci U S A Date: 2007-12-17 Impact factor: 11.205

10 in total

1. MicroC(3): an ex vivo microfluidic cis-coculture assay to test chemosensitivity and resistance of patient multiple myeloma cells.

Authors: Chorom Pak; Natalie S Callander; Edmond W K Young; Benjamin Titz; KyungMann Kim; Sandeep Saha; Kenny Chng; Fotis Asimakopoulos; David J Beebe; Shigeki Miyamoto
Journal: Integr Biol (Camb) Date: 2015-05-22 Impact factor: 2.192

Using a microfluidic device for high-content analysis of cell signaling.

1. Monolithic microfabricated valves and pumps by multilayer soft lithography.

2. Microfluidic large-scale integration.

3. Causal protein-signaling networks derived from multiparameter single-cell data.

4. Comment on "Oscillations in NF-kappaB signaling control the dynamics of gene expression".

5. A systems model of signaling identifies a molecular basis set for cytokine-induced apoptosis.

6. A plate reader-compatible microchannel array for cell biology assays.

7. The use of a charge-coupled device for quantitative optical microscopy of biological structures.

8. The response of human epithelial cells to TNF involves an inducible autocrine cascade.

9. Single cell profiling of potentiated phospho-protein networks in cancer cells.

10. Enhanced sensitivity to IGF-II signaling links loss of imprinting of IGF2 to increased cell proliferation and tumor risk.

1. MicroC(3): an ex vivo microfluidic cis-coculture assay to test chemosensitivity and resistance of patient multiple myeloma cells.

2. High-throughput microfluidic single-cell analysis pipeline for studies of signaling dynamics.

Review 3. Technology advancement for integrative stem cell analyses.

Review 4. High-content screening in microfluidic devices.

Review 5. Signal transduction: From the atomic age to the post-genomic era.

Review 6. Micro- and nanoengineering for stem cell biology: the promise with a caution.

7. High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity.

Review 8. Microfluidics-assisted in vitro drug screening and carrier production.

9. Sequential array cytometry: multi-parameter imaging with a single fluorescent channel.

10. Microfluidic reactors for advancing the MS analysis of fast biological responses.