| Literature DB >> 19531359 |
Yuji Nakayama1, Jessica M Shivas, Daniel S Poole, Jayne M Squirrell, Jennifer M Kulkoski, Justin B Schleede, Ahna R Skop.
Abstract
Cell polarity is crucial for the generation of cell diversity. Recent evidence suggests that the actin cytoskeleton plays a key role in establishment of embryonic polarity, yet the mechanisms that maintain polarity cues in particular membrane domains during development remain unclear. Dynamin, a large GTPase, functions in both endocytosis and actin dynamics. Here, the Caenorhabditis elegans dynamin ortholog, DYN-1, maintains anterior polarity cues. DYN-1-GFP foci are enriched in the anterior cortex in a manner dependent on the anterior polarity proteins, PAR-6 and PKC-3. Membrane internalization and actin comet formation are enriched in the anterior, and are dependent on DYN-1. PAR-6-labeled puncta are also internalized from cortical accumulations of DYN-1-GFP. Our results demonstrate a mechanism for the spatial and temporal regulation of endocytosis in the anterior of the embryo, contributing to the precise localization and maintenance of polarity factors within a dynamic plasma membrane.Entities:
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Year: 2009 PMID： 19531359 PMCID： PMC2719978 DOI： 10.1016/j.devcel.2009.04.009
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 12.270