M Xu1, H Zhou, J Wang, C Li, Y Yu. 1. Department of Endocrinology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Abstract
BACKGROUND: ATP-binding cassette transporters (ABCA1, ABCG1) and scavenger receptor class B type I (SR-BI) are the three most important cellular cholesterol transporters/receptor in regulating cholesterol efflux. We have investigated whether the expression of these transporters/receptor is altered in overweight and obese patients, and the potential association with the circulating adipokines. METHODS: Adiponectin, leptin and resistin were assayed in two groups of healthy controls, overweight and obese patients. The expression of ABCA1, ABCG1 and SR-BI in monocytes was measured. Cholesterol efflux from monocyte-derived macrophages was also determined. RESULTS: Circulating adiponectin was decreased, whereas leptin and resistin were increased in overweight and obese patients compared with healthy controls. ABCA1 expression was significantly decreased in overweight and obese patients (P<0.01), whereas the levels of ABCG1 and SR-BI were comparable between the two groups. Adiponectin was correlated with ABCA1 (r=0.44, P<0.001), but not with ABCG1 and SR-BI. No associations between leptin, resistin and the expression of ABCA1, ABCG1 and SR-BI were found. Cholesterol efflux from monocyte-derived macrophages to apolipoprotein AI or to autologous serum was significantly impaired in overweight and obese patients, which were correlated with ABCA1 expression (r=0.47, P<0.001; r=0.43, P<0.001, respectively). CONCLUSION: The expression of ABCA1 in monocytes is reduced in overweight and obese patients, and the reduction in ABCA1 is associated with the impairment of cholesterol efflux from monocyte-derived macrophages.
BACKGROUND: ATP-binding cassette transporters (ABCA1, ABCG1) and scavenger receptor class B type I (SR-BI) are the three most important cellular cholesterol transporters/receptor in regulating cholesterol efflux. We have investigated whether the expression of these transporters/receptor is altered in overweight and obesepatients, and the potential association with the circulating adipokines. METHODS:Adiponectin, leptin and resistin were assayed in two groups of healthy controls, overweight and obesepatients. The expression of ABCA1, ABCG1 and SR-BI in monocytes was measured. Cholesterol efflux from monocyte-derived macrophages was also determined. RESULTS: Circulating adiponectin was decreased, whereas leptin and resistin were increased in overweight and obesepatients compared with healthy controls. ABCA1 expression was significantly decreased in overweight and obesepatients (P<0.01), whereas the levels of ABCG1 and SR-BI were comparable between the two groups. Adiponectin was correlated with ABCA1 (r=0.44, P<0.001), but not with ABCG1 and SR-BI. No associations between leptin, resistin and the expression of ABCA1, ABCG1 and SR-BI were found. Cholesterol efflux from monocyte-derived macrophages to apolipoprotein AI or to autologous serum was significantly impaired in overweight and obesepatients, which were correlated with ABCA1 expression (r=0.47, P<0.001; r=0.43, P<0.001, respectively). CONCLUSION: The expression of ABCA1 in monocytes is reduced in overweight and obesepatients, and the reduction in ABCA1 is associated with the impairment of cholesterol efflux from monocyte-derived macrophages.
Authors: Manal S Fawzy; Osama Alhadramy; Mohammad H Hussein; Hussein M Ismail; Nesreen M Ismail; Nouran M Biomy; Eman A Toraih Journal: Mol Diagn Ther Date: 2015-08 Impact factor: 4.074
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