Literature DB >> 19524011

The influence of different glycosylation patterns on factor VII biological activity.

Ieva Sutkeviciute1, Edita Mistiniene, Jolanta Sereikaite, Vladas A Bumelis.   

Abstract

In this study the bioactivity of three differently glycosylated blood coagulation factor VII (FVII) variants (human plasma FVII, recombinant human FVII produced in CHO and BHK cell cultures) were analyzed and compared. Surface plasmon resonance studies of FVII interaction with soluble and full length TF together with FVII autoactivation assays revealed that BHK-derived FVII has the highest bioactivity, while human plasma and CHO-derived FVII showed very similar bioactivity. The affinity of FVII variants to TF correlates with FVII autoactivation rates--the higher the affinity, the faster the autoactivation rate.

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Year:  2009        PMID： 19524011     DOI： 10.1016/j.biochi.2009.05.015

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  4 in total

Review 1.  Effects of Glycosylation on the Enzymatic Activity and Mechanisms of Proteases.

Authors:  Peter Goettig
Journal:  Int J Mol Sci       Date:  2016-11-25       Impact factor: 5.923

2.  Functional and Molecular Characterization of C91S Mutation in the Second Epidermal Growth Factor-Like Domain of Factor VII.

Authors:  Amir Mashayekhi; Shirin Shahbazi; Mirdavood Omrani
Journal:  Iran J Biotechnol       Date:  2018-04-18       Impact factor: 1.671

3.  In vitro characterization of CT-001-a short-acting factor VIIa with enhanced prohemostatic activity.

Authors:  Derek S Sim; Cornell R Mallari; John M Teare; Richard I Feldman; Maxine Bauzon; Terry W Hermiston
Journal:  Res Pract Thromb Haemost       Date:  2021-06-30

4.  Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells.

Authors:  Ernst Böhm; Birgit K Seyfried; Michael Dockal; Michael Graninger; Meinhard Hasslacher; Marianne Neurath; Christian Konetschny; Peter Matthiessen; Artur Mitterer; Friedrich Scheiflinger
Journal:  BMC Biotechnol       Date:  2015-09-18       Impact factor: 2.563
  4 in total

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