AIM: To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma. METHODS: Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma, its corresponding non-neoplastic mucosa, and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR and Western blotting. RESULTS: The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa, primary gastric carcinoma and lymph node metastasis (P < 0.05). Moreover, the expression of semaphorin 5A was closely correlated with that of plexin B3. CONCLUSION: Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.
AIM: To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma. METHODS: Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma, its corresponding non-neoplastic mucosa, and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR and Western blotting. RESULTS: The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa, primary gastric carcinoma and lymph node metastasis (P < 0.05). Moreover, the expression of semaphorin 5A was closely correlated with that of plexin B3. CONCLUSION:Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.
Authors: David B Kantor; Onanong Chivatakarn; Katherine L Peer; Stephen F Oster; Masaru Inatani; Michael J Hansen; John G Flanagan; Yu Yamaguchi; David W Sretavan; Roman J Giger; Alex L Kolodkin Journal: Neuron Date: 2004-12-16 Impact factor: 17.173
Authors: Stefania Artigiani; Paolo Conrotto; Pietro Fazzari; Giorgio F Gilestro; Davide Barberis; Silvia Giordano; Paolo M Comoglio; Luca Tamagnone Journal: EMBO Rep Date: 2004-06-25 Impact factor: 8.807
Authors: Jeffrey L Goldberg; Mauricio E Vargas; Jack T Wang; Wim Mandemakers; Stephen F Oster; David W Sretavan; Ben A Barres Journal: J Neurosci Date: 2004-05-26 Impact factor: 6.167
Authors: Lea H Gregersen; Anders Jacobsen; Lisa B Frankel; Jiayu Wen; Anders Krogh; Anders H Lund Journal: BMC Cancer Date: 2012-06-12 Impact factor: 4.430
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