| Literature DB >> 19520133 |
Chang-Suk Kong1, Jung-Ae Kim, Se-Kwon Kim.
Abstract
In this study, we investigated the effect of sulfated glucosamine (SGlc) on adipogenesis of 3T3-L1 adipocytes during differentiation of preadipocytes into adipocytes by measuring lipid accumulation and adipogenesis related factors. Treatment with SGlc reduced the triglyceride content in Oil-Red O staining and enhanced glycerol secretion in adipocytes in a dose-dependent manner. In addition, SGlc induced the down-regulation of adipogenesis related factors and adipocyte specific gene promoters. Moreover, treatment of 3T3-L1 adipocytes with SGlc activated the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) alpha and beta along with their substrate, acetyl-CoA carboxylase (ACC). These results suggest that inhibitory effect of SGlc on adipocyte differentiation might be mediated through the up-regulation of AMPK pathway.Entities:
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Year: 2009 PMID： 19520133 DOI： 10.1016/j.fct.2009.06.010
Source DB: PubMed Journal: Food Chem Toxicol ISSN： 0278-6915 Impact factor: 6.023