Literature DB >> 19519853

Effect of different sample volumes on the DNA extraction of Aspergillus fumigatus from whole blood.

B Schulz1, K Weber, C Radecke, C Scheer, M Ruhnke.   

Abstract

Five methods were compared, using conventional PCR, for the isolation of DNA from Aspergillus fumigatus conidia from 1-3-mL samples of whole blood. A lower detection threshold of Aspergillus conidia was achieved using 3-mL rather than 1-mL samples with three of five methods tested.

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Year:  2009        PMID: 19519853     DOI: 10.1111/j.1469-0691.2009.02797.x

Source DB:  PubMed          Journal:  Clin Microbiol Infect        ISSN: 1198-743X            Impact factor:   8.067


  1 in total

1.  Aspergillus PCR: one step closer to standardization.

Authors:  P Lewis White; Stéphane Bretagne; Lena Klingspor; Willem J G Melchers; Elaine McCulloch; Bettina Schulz; Niklas Finnstrom; Carlo Mengoli; Rosemary A Barnes; J Peter Donnelly; Juergen Loeffler
Journal:  J Clin Microbiol       Date:  2010-02-10       Impact factor: 5.948

  1 in total

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