Literature DB >> 19518143

Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis.

Linas Mazutis1, Ali Fallah Araghi, Oliver J Miller, Jean-Christophe Baret, Lucas Frenz, Agnes Janoshazi, Valérie Taly, Benjamin J Miller, J Brian Hutchison, Darren Link, Andrew D Griffiths, Michael Ryckelynck.   

Abstract

We have developed a method for high-throughput isothermal amplification of single DNA molecules in a droplet-based microfluidic system. DNA amplification in droplets was analyzed using an intercalating fluorochrome, allowing fast and accurate "digital" quantification of the template DNA based on the Poisson distribution of DNA molecules in droplets. The clonal amplified DNA in each 2 pL droplet was further analyzed by measuring the enzymatic activity of the encoded proteins after fusion with a 15 pL droplet containing an in vitro translation system.

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Year:  2009        PMID： 19518143     DOI： 10.1021/ac900403z

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  57 in total

1.  Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCR.

Authors:  Jason E Kreutz; Todd Munson; Toan Huynh; Feng Shen; Wenbin Du; Rustem F Ismagilov
Journal:  Anal Chem       Date:  2011-10-07       Impact factor: 6.986

Review 2.  Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.

Authors:  Aniruddha M Kaushik; Kuangwen Hsieh; Tza-Huei Wang
Journal:  Wiley Interdiscip Rev Nanomed Nanobiotechnol       Date:  2018-05-24

3.  Droplet-based microfluidic washing module for magnetic particle-based assays.

Authors:  Hun Lee; Linfeng Xu; Kwang W Oh
Journal:  Biomicrofluidics       Date:  2014-08-01       Impact factor: 2.800

4.  On demand nanoliter-scale microfluidic droplet generation, injection, and mixing using a passive microfluidic device.

Authors:  Uwe Tangen; Abhishek Sharma; Patrick Wagler; John S McCaskill
Journal:  Biomicrofluidics       Date:  2015-02-12       Impact factor: 2.800

5.  Digital PCR on a SlipChip.

Authors:  Feng Shen; Wenbin Du; Jason E Kreutz; Alice Fok; Rustem F Ismagilov
Journal:  Lab Chip       Date:  2010-07-01       Impact factor: 6.799

6.  Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction.

Authors:  Charline J J Gerard; Gilles Ferry; Laurent M Vuillard; Jean A Boutin; Leonard M G Chavas; Tiphaine Huet; Nathalie Ferte; Romain Grossier; Nadine Candoni; Stéphane Veesler
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2017-10-02       Impact factor: 1.056

7.  Massively parallel detection of gene expression in single cells using subnanolitre wells.

Authors:  Yuan Gong; Adebola O Ogunniyi; J Christopher Love
Journal:  Lab Chip       Date:  2010-08-04       Impact factor: 6.799

8.  Digital LAMP in a sample self-digitization (SD) chip.

Authors:  Alexander Gansen; Alison M Herrick; Ivan K Dimov; Luke P Lee; Daniel T Chiu
Journal:  Lab Chip       Date:  2012-03-07       Impact factor: 6.799

Review 9.  Advances in microfluidic PCR for point-of-care infectious disease diagnostics.

Authors:  Seungkyung Park; Yi Zhang; Shin Lin; Tza-Huei Wang; Samuel Yang
Journal:  Biotechnol Adv       Date:  2011-06-30       Impact factor: 14.227

10.  Quantifying phenotypes in single cells using droplet microfluidics.

Authors:  Fengjiao Lyu; Lucas R Blauch; Sindy K Y Tang
Journal:  Methods Cell Biol       Date:  2018-10-25       Impact factor: 1.441
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