Y-Y Liu1, S-x Guo. 1. Laboratory of Medicinal Fungi, Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Abstract
AIMS: To find out which nutritional condition is the determining factor for sclerotial formation of Polyporus umbellatus. METHODS AND RESULTS: The nutritional requirements of 15 carbohydrates, ten nitrogen compounds, eight vitamins and eight mineral elements were studied for their effects on mycelial growth and sclerotial formation of Polyporus umbellatus using the one-factor-at-a-time method. Only fructose could induce sclerotial formation of P. umbellatus. An additional test indicated that nitrogen source categories influenced sclerotial formation significantly and that peptone was found to be the best for sclerotial production. Through an orthogonal matrix test, the effects of carbon/nitrogen factors on sclerotial formation were found be in the order: fructose > interaction between fructose and peptone > peptone. The optimal concentration for sclerotial formation was determined to be 50.0 g l(-1) fructose and 4.0 g l(-1) peptone. CONCLUSIONS: Carbon source is the factor determining sclerotial formation of Polyporus umbellatus. Nitrogen source can influence such a morphological transformation significantly. The categories of vitamin and mineral element do not have relationship with the sclerotial formation. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides the preparatory knowledge for the completely artificial culture of Polyporus umbellatus for its sclerotium.
AIMS: To find out which nutritional condition is the determining factor for sclerotial formation of Polyporus umbellatus. METHODS AND RESULTS: The nutritional requirements of 15 carbohydrates, ten nitrogen compounds, eight vitamins and eight mineral elements were studied for their effects on mycelial growth and sclerotial formation of Polyporus umbellatus using the one-factor-at-a-time method. Only fructose could induce sclerotial formation of P. umbellatus. An additional test indicated that nitrogen source categories influenced sclerotial formation significantly and that peptone was found to be the best for sclerotial production. Through an orthogonal matrix test, the effects of carbon/nitrogen factors on sclerotial formation were found be in the order: fructose > interaction between fructose and peptone > peptone. The optimal concentration for sclerotial formation was determined to be 50.0 g l(-1) fructose and 4.0 g l(-1) peptone. CONCLUSIONS:Carbon source is the factor determining sclerotial formation of Polyporus umbellatus. Nitrogen source can influence such a morphological transformation significantly. The categories of vitamin and mineral element do not have relationship with the sclerotial formation. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides the preparatory knowledge for the completely artificial culture of Polyporus umbellatus for its sclerotium.