BACKGROUND: Cell transfection with nanoscaled cationic polymeric particles using Chitosan has been extensively explored. Because of its properties such as cationic charges, biocompatibility, biodegradability, and low toxicity, it has been used as a potential gene, siRNA, protein (including antibodies), and drug carrier system. METHOD: This work describes the development of chitosan nanoparticles of a 20-nm diameter for a potential siRNA delivery application. The particles were prepared using an ionic gelation method, using sodium tripolyphosphate as a cross-linker. The effect of variation in pH was investigated on particle size and surface charge. Gene loading efficiency by chitosan nanoparticles was performed by varying weight ratios of chitosan: siRNA. Transfection efficiency was evaluated on Neuro2a cells. RESULTS: It was observed that 20-nm-sized nanoscale complexes induced significant transfection in neuronal cells. CONCLUSION: These particles have potential in the delivery of siRNA to neural tissues.
BACKGROUND: Cell transfection with nanoscaled cationic polymeric particles using Chitosan has been extensively explored. Because of its properties such as cationic charges, biocompatibility, biodegradability, and low toxicity, it has been used as a potential gene, siRNA, protein (including antibodies), and drug carrier system. METHOD: This work describes the development of chitosan nanoparticles of a 20-nm diameter for a potential siRNA delivery application. The particles were prepared using an ionic gelation method, using sodium tripolyphosphate as a cross-linker. The effect of variation in pH was investigated on particle size and surface charge. Gene loading efficiency by chitosan nanoparticles was performed by varying weight ratios of chitosan: siRNA. Transfection efficiency was evaluated on Neuro2a cells. RESULTS: It was observed that 20-nm-sized nanoscale complexes induced significant transfection in neuronal cells. CONCLUSION: These particles have potential in the delivery of siRNA to neural tissues.