Literature DB >> 19513671

Purification strategy for recombinant forms of the human mitochondrial DNA helicase.

Tawn D Ziebarth1, Laurie S Kaguni.   

Abstract

In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification of this enzyme facilitates studies of its structure and mechanism and allows elucidation of native features of both wild-type- and human disease-related forms.

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Year:  2009        PMID： 19513671      PMCID： PMC4703102          DOI： 10.1007/978-1-59745-521-3_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

Review 1.  Helicase structure and mechanism.

Authors:  Jonathan M Caruthers; David B McKay
Journal:  Curr Opin Struct Biol       Date:  2002-02       Impact factor: 6.809

2.  Modular architecture of the hexameric human mitochondrial DNA helicase.

Authors:  Tawn D Ziebarth; Carol L Farr; Laurie S Kaguni
Journal:  J Mol Biol       Date:  2007-02-07       Impact factor: 5.469

3.  TWINKLE Has 5' -> 3' DNA helicase activity and is specifically stimulated by mitochondrial single-stranded DNA-binding protein.

Authors:  Jenny A Korhonen; Martina Gaspari; Maria Falkenberg
Journal:  J Biol Chem       Date:  2003-09-15       Impact factor: 5.157

4.  Human mitochondrial DNA deletions associated with mutations in the gene encoding Twinkle, a phage T7 gene 4-like protein localized in mitochondria.

Authors:  J N Spelbrink; F Y Li; V Tiranti; K Nikali; Q P Yuan; M Tariq; S Wanrooij; N Garrido; G Comi; L Morandi; L Santoro; A Toscano; G M Fabrizi; H Somer; R Croxen; D Beeson; J Poulton; A Suomalainen; H T Jacobs; M Zeviani; C Larsson
Journal:  Nat Genet       Date:  2001-07       Impact factor: 38.330

5.  Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.

Authors:  J E Walker; M Saraste; M J Runswick; N J Gay
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

6.  Twinkle helicase is essential for mtDNA maintenance and regulates mtDNA copy number.

Authors:  Henna Tyynismaa; Hiroshi Sembongi; Monika Bokori-Brown; Caroline Granycome; Neil Ashley; Joanna Poulton; Anu Jalanko; Johannes N Spelbrink; Ian J Holt; Anu Suomalainen
Journal:  Hum Mol Genet       Date:  2004-10-27       Impact factor: 6.150
  6 in total
  2 in total

1.  Physical analysis of recombinant forms of the human mitochondrial DNA helicase.

Authors:  Magdalena M Makowska-Grzyska; Tawn D Ziebarth; Laurie S Kaguni
Journal:  Methods       Date:  2010-03-25       Impact factor: 3.608

2.  Purification and Comparative Assay of the Human Mitochondrial Replicative DNA Helicase.

Authors:  Fernando A Rosado-Ruiz; Minyoung So; Laurie S Kaguni
Journal:  Methods Mol Biol       Date:  2016
  2 in total

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