INTRODUCTION: Cyclin D1 has been implicated in the progression of several cancers by virtue of its influence on progression of the G1/S phase of the cell cycle. However, little is known about the possible roles of cyclin D2 and D3 in colorectal cancers (CRCs). METHOD: We investigated the expression levels of cyclin D2 and D3 in 84 CRC specimens. Antigen expression was determined by immunohistochemical analysis of cyclin D1, D2, D3, p16INK4A and Ki67 on tissue microarrays constructed using core samples from tumour centres and margins. RESULTS: For the whole cohort, expression of cyclin D2 at the margin was associated with vascular invasion (P = 0.039), lymph node metastasis (P = 0.020) and liver metastasis (P < 0.001). In patients with stage I and II tumours (n = 84), elevated cyclin D2 and D3 were associated with vascular invasion (P = 0.014 and 0.028 respectively), liver metastasis (P = 0.001 and 0.007 respectively) and reduced disease specific survival (Cyclin D2, P < 0.022). No association was noted between the proliferative marker Ki-67 and the D-type cyclins. CONCLUSION: These findings suggest that cyclin D2 expression at the invasive margin of CRCs is associated with liver metastasis and may serve as a useful prognostic marker and indicator of the need for adjuvant therapy.
INTRODUCTION:Cyclin D1 has been implicated in the progression of several cancers by virtue of its influence on progression of the G1/S phase of the cell cycle. However, little is known about the possible roles of cyclin D2 and D3 in colorectal cancers (CRCs). METHOD: We investigated the expression levels of cyclin D2 and D3 in 84 CRC specimens. Antigen expression was determined by immunohistochemical analysis of cyclin D1, D2, D3, p16INK4A and Ki67 on tissue microarrays constructed using core samples from tumour centres and margins. RESULTS: For the whole cohort, expression of cyclin D2 at the margin was associated with vascular invasion (P = 0.039), lymph node metastasis (P = 0.020) and liver metastasis (P < 0.001). In patients with stage I and II tumours (n = 84), elevated cyclin D2 and D3 were associated with vascular invasion (P = 0.014 and 0.028 respectively), liver metastasis (P = 0.001 and 0.007 respectively) and reduced disease specific survival (Cyclin D2, P < 0.022). No association was noted between the proliferative marker Ki-67 and the D-type cyclins. CONCLUSION: These findings suggest that cyclin D2 expression at the invasive margin of CRCs is associated with liver metastasis and may serve as a useful prognostic marker and indicator of the need for adjuvant therapy.
Authors: Maria Schwaederlé; Gregory A Daniels; David E Piccioni; Paul T Fanta; Richard B Schwab; Kelly A Shimabukuro; Barbara A Parker; Razelle Kurzrock Journal: Oncotarget Date: 2015-02-20
Authors: Nicola Whiffin; Fay J Hosking; Susan M Farrington; Claire Palles; Sara E Dobbins; Lina Zgaga; Amy Lloyd; Ben Kinnersley; Maggie Gorman; Albert Tenesa; Peter Broderick; Yufei Wang; Ella Barclay; Caroline Hayward; Lynn Martin; Daniel D Buchanan; Aung Ko Win; John Hopper; Mark Jenkins; Noralane M Lindor; Polly A Newcomb; Steve Gallinger; David Conti; Fred Schumacher; Graham Casey; Tao Liu; Harry Campbell; Annika Lindblom; Richard S Houlston; Ian P Tomlinson; Malcolm G Dunlop Journal: Hum Mol Genet Date: 2014-04-15 Impact factor: 6.150