PURPOSE: This study is designed to investigate the roles of Tiam1 and Rac1 in nasopharyngeal carcinoma (NPC). METHODS: NPC samples (n = 102) were analyzed with immunohistochemistry for Tiam1 and Rac1 proteins, 28 of which were also analyzed with quantitative RT-PCR and Western blots for Tiam1 and Rac1 mRNA and protein expression. The expression of Tiam1 and Rac1 in noncancerous nasopharyngeal tissue (n = 26) acted as a control. The expression was gauged regarding stage, grade and survival. RESULTS: Tiam1 and Rac1 were overexpressed in NPC cells and their protein upregulation was significantly associated with stage and grading (p < 0.05 for all). Cox regression analysis revealed Tiam1 and Rac1 protein upregulation was correlated with lower disease-free and overall survival rates (p < 0.001). CONCLUSIONS: Upregulation of Tiam1 and Rac1 proteins may play a critical role in tumor progression of NPC, and work as a prognostic factor for NPC patients.
PURPOSE: This study is designed to investigate the roles of Tiam1 and Rac1 in nasopharyngeal carcinoma (NPC). METHODS: NPC samples (n = 102) were analyzed with immunohistochemistry for Tiam1 and Rac1 proteins, 28 of which were also analyzed with quantitative RT-PCR and Western blots for Tiam1 and Rac1 mRNA and protein expression. The expression of Tiam1 and Rac1 in noncancerous nasopharyngeal tissue (n = 26) acted as a control. The expression was gauged regarding stage, grade and survival. RESULTS:Tiam1 and Rac1 were overexpressed in NPC cells and their protein upregulation was significantly associated with stage and grading (p < 0.05 for all). Cox regression analysis revealed Tiam1 and Rac1 protein upregulation was correlated with lower disease-free and overall survival rates (p < 0.001). CONCLUSIONS: Upregulation of Tiam1 and Rac1 proteins may play a critical role in tumor progression of NPC, and work as a prognostic factor for NPC patients.
Authors: Natalie A Mack; Andrew P Porter; Helen J Whalley; Juliane P Schwarz; Richard C Jones; Azharuddin Sajid Syed Khaja; Anders Bjartell; Kurt I Anderson; Angeliki Malliri Journal: Nat Cell Biol Date: 2012-10-28 Impact factor: 28.824