Literature DB >> 19504264

Autometallographic enhancement of the Golgi-Cox staining enables high resolution visualization of dendrites and spines.

Dariusz Orlowski1, Carsten R Bjarkam.   

Abstract

We present a method for autometallographic (AMG) enhancement of the Golgi-Cox staining enabling high resolution visualization of dendrites and spines. The method is cheaper and more flexible than conventional enhancement procedures performed with commercial photographic developers. The staining procedure is thoroughly described and we demonstrate with qualitative and quantitative data, how histological tissue sectioning, Golgi-Cox immersion time and different AMG enhancement length may influence the staining of dendrites and spines in the rat hippocampus. The described method will be of value for future behavioural-anatomical studies, examining changes in dendrite branching and spine density caused by brain diseases and their subsequent treatment.

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Year:  2009        PMID： 19504264     DOI： 10.1007/s00418-009-0611-3

Source DB:  PubMed          Journal:  Histochem Cell Biol        ISSN： 0948-6143            Impact factor:   4.304


  28 in total

1.  Histochemical differentiation of autometallographically traceable metals (Au, Ag, Hg, Bi, Zn): protocols for chemical removal of separate autometallographic metal clusters in Epon sections.

Authors:  M Stoltenberg; G Danscher
Journal:  Histochem J       Date:  2000-11

Review 2.  Silver enhancement of quantum dots resulting from (1) metabolism of toxic metals in animals and humans, (2) in vivo, in vitro and immersion created zinc-sulphur/zinc-selenium nanocrystals, (3) metal ions liberated from metal implants and particles.

Authors:  Gorm Danscher; Meredin Stoltenberg
Journal:  Prog Histochem Cytochem       Date:  2006-08-07

3.  A rapid method combining Golgi and Nissl staining to study neuronal morphology and cytoarchitecture.

Authors:  Nadia Pilati; Matthew Barker; Sofoklis Panteleimonitis; Revers Donga; Martine Hamann
Journal:  J Histochem Cytochem       Date:  2008-02-18       Impact factor: 2.479

4.  The actions of BDNF on dendritic spine density and morphology in organotypic slice cultures depend on the presence of serum in culture media.

Authors:  Christopher A Chapleau; Maria E Carlo; Jennifer L Larimore; Lucas Pozzo-Miller
Journal:  J Neurosci Methods       Date:  2007-12-23       Impact factor: 2.390

5.  Automated quantification of dendritic spine density and spine head diameter in medium spiny neurons of the nucleus accumbens.

Authors:  Haowei Shen; Susan R Sesack; Shigenobu Toda; Peter W Kalivas
Journal:  Brain Struct Funct       Date:  2008-06-06       Impact factor: 3.270

6.  On the "selectivity" of the Golgi-Cox method.

Authors:  J F Pasternak; T A Woolsey
Journal:  J Comp Neurol       Date:  1975-04-01       Impact factor: 3.215

7.  Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys.

Authors:  Gorazd Rosoklija; Branislav Mancevski; Boro Ilievski; Tarique Perera; Sarah H Lisanby; Jeremy D Coplan; Aleksej Duma; Tereza Serafimova; Andrew J Dwork
Journal:  J Neurosci Methods       Date:  2003-12-30       Impact factor: 2.390

8.  Diversity and fluctuation of spine morphology in CA1 pyramidal neurons after transient global ischemia.

Authors:  Yi-Wen Ruan; Zhigang Lei; Yuan Fan; Bende Zou; Zao C Xu
Journal:  J Neurosci Res       Date:  2009-01       Impact factor: 4.164

9.  Autometallography. A new technique for light and electron microscopic visualization of metals in biological tissues (gold, silver, metal sulphides and metal selenides).

Authors:  G Danscher
Journal:  Histochemistry       Date:  1984

10.  SUMOylation of the MAGUK protein CASK regulates dendritic spinogenesis.

Authors:  Hsu-Wen Chao; Chen-Jei Hong; Tzyy-Nan Huang; Yi-Ling Lin; Yi-Ping Hsueh
Journal:  J Cell Biol       Date:  2008-07-07       Impact factor: 10.539
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  3 in total

Review 1.  Extending the knowledge in histochemistry and cell biology.

Authors:  Wolfgang-Moritz Heupel; Detlev Drenckhahn
Journal:  Histochem Cell Biol       Date:  2009-11-28       Impact factor: 4.304

2.  Simultaneous acquisition of neuronal morphology and cytoarchitecture in the same Golgi-stained brain.

Authors:  Xiao Chen; Xiaoyu Zhang; Qiuyuan Zhong; Qingtao Sun; Jie Peng; Hui Gong; Jing Yuan
Journal:  Biomed Opt Express       Date:  2017-12-18       Impact factor: 3.732

3.  Neurite density from magnetic resonance diffusion measurements at ultrahigh field: comparison with light microscopy and electron microscopy.

Authors:  Sune N Jespersen; Carsten R Bjarkam; Jens R Nyengaard; M Mallar Chakravarty; Brian Hansen; Thomas Vosegaard; Leif Østergaard; Dmitriy Yablonskiy; Niels Chr Nielsen; Peter Vestergaard-Poulsen
Journal:  Neuroimage       Date:  2009-09-02       Impact factor: 6.556
  3 in total

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