| Literature DB >> 19504068 |
Wendy J K Gardiner1, Lydia Teboul.
Abstract
Pronuclear injection is a mode of DNA delivery for the production of transgenic animals. We present protocols for the preparation of DNA for microinjection from small or large plasmids and from BACs. We describe the production of one-cell mouse embryos, unfertile males, and pseudo-pregnant females. Finally, we detail the set up and procedures for the microinjection of one-cell embryos and their transfer into foster females.Entities:
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Year: 2009 PMID： 19504068 DOI： 10.1007/978-1-60327-019-9_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745