Literature DB >> 19499708

Collagenase isoforms for pancreas digestion.

Federico Bertuzzi1, Silvia Cainarca, Simona Marzorati, Angela Bachi, Barbara Antonioli, Rita Nano, Roberto Verzaro, Camillo Ricordi.   

Abstract

The available information concerning the characteristics and composition of collagenase batches, which are effective in the digestion of human pancreas for islet transplants, is scarce and incomplete. A large inter- and intrabatched variability in activity and efficiency of blend enzymes available for isolation has been observed. The aim of this study was to characterize enzyme blend components. Liberase batches were characterized by SDS-PAGE analyses, microelectrophoresis, and then by MALDI-TOF MS analysis. Three main bands were detected by SDS-PAGE analysis and submitted to MALDI-TOF MS analysis. Two bands were found to correspond to class I (isoform beta and another of 106 kDa) and one to class II (isoform delta) collagenase. These results represent an important step towards a complete characterization of enzymes, with the final aim of identifying key components for a standardized product.

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Year:  2009        PMID： 19499708     DOI： 10.3727/096368909788341270

Source DB:  PubMed          Journal:  Cell Transplant        ISSN： 0963-6897            Impact factor:   4.064


  7 in total

1.  A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products.

Authors:  A N Balamurugan; Gopalakrishnan Loganathan; Melena D Bellin; Joshua J Wilhelm; James Harmon; Takayuki Anazawa; Sajjad M Soltani; David M Radosevich; Takeshi Yuasa; Mukesh Tiwari; Klearchos K Papas; Robert McCarthy; David E R Sutherland; Bernhard J Hering
Journal:  Transplantation       Date:  2012-04-15       Impact factor: 4.939

2.  Different digestion enzymes used for human pancreatic islet isolation: a mixed treatment comparison (MTC) meta-analysis.

Authors:  Jakeline Rheinheimer; Patrícia Klarmann Ziegelmann; Rodrigo Carlessi; Luciana Ross Reck; Andrea Carla Bauer; Cristiane Bauermann Leitão; Daisy Crispim
Journal:  Islets       Date:  2014-11-07       Impact factor: 2.694

3.  The Choice of Enzyme for Human Pancreas Digestion is a Critical Factor for Increasing the Success of Islet Isolation.

Authors:  Meirigeng Qi; Luis Valiente; Brian McFadden; Keiko Omori; Shiela Bilbao; Jemily Juan; Jeffrey Rawson; Stephen Scott; Kevin Ferreri; Yoko Mullen; Mohamed El-Shahawy; Donald Dafoe; Fouad Kandeel; Ismail H Al-Abdullah
Journal:  Transplant Direct       Date:  2015-05

4.  Evaluation of viable β-cell mass is useful for selecting collagenase for human islet isolation: comparison of collagenase NB1 and liberase HI.

Authors:  R Misawa; C Ricordi; A Miki; S Barker; R D Molano; A Khan; S Miyagawa; L Inverardi; R Alejandro; A Pileggi; H Ichii
Journal:  Cell Transplant       Date:  2011-09-16       Impact factor: 4.064

5.  Multicenter analysis of novel and established variables associated with successful human islet isolation outcomes.

Authors:  J S Kaddis; J S Danobeitia; J C Niland; T Stiller; L A Fernandez
Journal:  Am J Transplant       Date:  2010-01-05       Impact factor: 8.086

6.  Fluorogenic Peptide Substrate for Quantification of Bacterial Enzyme Activities.

Authors:  Ismail H Al-Abdullah; Karine Bagramyan; Shiela Bilbao; Meirigeng Qi; Markus Kalkum
Journal:  Sci Rep       Date:  2017-03-13       Impact factor: 4.379

7.  Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata.

Authors:  Bashar Khiatah; Amber Tucker; Kuan-Tsen Chen; Rachel Perez; Shiela Bilbao; Luis Valiente; Leonard Medrano; Jeffrey Rawson; Elena Forouhar; Keiko Omori; Fouad Kandeel; Meirigeng Qi; Ismail H Al-Abdullah
Journal:  Islets       Date:  2018-02-02       Impact factor: 2.694
  7 in total

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