OBJECTIVE: To examine the molecular epidemiology of syphilis in Scotland. METHODS: Ulcer specimens were collected from 85 patients with infectious syphilis. Typing of Treponema pallidum was performed using a method that examines variation in two loci; the number of 60-basepair repeats within the arp gene and sequence variation in the tpr genes. RESULTS: Patients were predominately white men who have sex with men (MSM). Treponemal DNA was detected in 75 specimens and a total of six subtypes were identified from 58 typeable specimens (77%). The most common subtypes were 14d (44/58, 76%), followed by 14e (7/58, 12%), 14j (3/58, 5%), 14b (2/58, 3%), 14p and 14k (1/58, 2%). CONCLUSIONS: This study shows that subtype 14d is the predominant subtype circulating in Scotland and there is a surprising level of genetic diversity within the Scottish MSM community.
OBJECTIVE: To examine the molecular epidemiology of syphilis in Scotland. METHODS: Ulcer specimens were collected from 85 patients with infectious syphilis. Typing of Treponema pallidum was performed using a method that examines variation in two loci; the number of 60-basepair repeats within the arp gene and sequence variation in the tpr genes. RESULTS:Patients were predominately white men who have sex with men (MSM). Treponemal DNA was detected in 75 specimens and a total of six subtypes were identified from 58 typeable specimens (77%). The most common subtypes were 14d (44/58, 76%), followed by 14e (7/58, 12%), 14j (3/58, 5%), 14b (2/58, 3%), 14p and 14k (1/58, 2%). CONCLUSIONS: This study shows that subtype 14d is the predominant subtype circulating in Scotland and there is a surprising level of genetic diversity within the Scottish MSM community.
Authors: P A Grange; L Gressier; P L Dion; D Farhi; N Benhaddou; P Gerhardt; J P Morini; J Deleuze; C Pantoja; A Bianchi; F Lassau; M F Avril; M Janier; N Dupin Journal: J Clin Microbiol Date: 2012-01-04 Impact factor: 5.948
Authors: Christina M Marra; Sharon K Sahi; Lauren C Tantalo; Charmie Godornes; Tara Reid; Frieda Behets; Anne Rompalo; Jeffrey D Klausner; Yue Ping Yin; Fiona Mulcahy; Matthew R Golden; Arturo Centurion-Lara; Sheila A Lukehart Journal: J Infect Dis Date: 2010-11-01 Impact factor: 5.226
Authors: Adriana R Cruz; Allan Pillay; Ana V Zuluaga; Lady G Ramirez; Jorge E Duque; Gloria E Aristizabal; Mary D Fiel-Gan; Roberto Jaramillo; Rodolfo Trujillo; Carlos Valencia; Linda Jagodzinski; David L Cox; Justin D Radolf; Juan C Salazar Journal: PLoS Negl Trop Dis Date: 2010-05-18