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Literature DB >> 19497391

Enhanced osteocalcin expression by osteoblast-like cells (MC3T3-E1) exposed to bioactive coating glass (SiO2-CaO-P2O5-MgO-K2O-Na2O system) ions.

V G Varanasi¹, E Saiz, P M Loomer, B Ancheta, N Uritani, S P Ho, A P Tomsia, S J Marshall, G W Marshall.

Abstract

This study tested the hypothesis that bioactive coating glass (SiO(2)-CaO-P(2)O(5)-MgO-K(2)O-Na(2)O system), used for implant coatings, enhanced the induction of collagen type 1 synthesis and in turn enhanced the expression of downstream markers alkaline phosphatase, Runx2 and osteocalcin during osteoblast differentiation. The ions from experimental bioactive glass (6P53-b) and commercial Bioglass(TM) (45S5) were added to osteoblast-like MC3T3-E1 subclone 4 cultures as a supplemented ion extract (glass conditioned medium (GCM)). Ion extracts contained significantly higher concentrations of Si and Ca (Si, 47.9+/-10.4 ppm; Ca, 69.8+/-14.0 for 45S5; Si, 33.4+/-3.8 ppm; Ca, 57.1+/-2.8 ppm for 6P53-b) compared with the control extract (Si<0.1 ppm, Ca 49.0 ppm in alpha-MEM) (ANOVA, p<0.05). Cell proliferation rate was enhanced (1.5x control) within the first 3 days after adding 45S5 and 6P53-b GCM. MC3T3-E1 subclone 4 cultures were then studied for their response to the addition of test media (GCM and control medium along with ascorbic acid (AA; 50 ppm)). Each GCM+AA treatment enhanced collagen type 1 synthesis as observed in both gene expression results (day 1, Col1alpha1, 45S5 GCM+AA: 3x control+AA; 6P53-b GCM+AA: 4x control+AA; day 5, Col1alpha2, 45S5 GCM+AA: 3.15x control+AA; 6P53-b GCM+AA: 2.35x control+AA) and in histological studies (Picrosirius stain) throughout the time course of early differentiation. Continued addition of each GCM and AA treatment led to enhanced expression of alkaline phosphatase (1.4x control+AA after 5 days, 2x control+AA after 10 days), Runx2 (2x control+AA after 7 days) and osteocalcin gene (day 3, 45S5 GCM+AA: 14x control+AA; day 5, 6P53-b GCM+AA: 19x control+AA) and protein expression (40x-70x control+AA after 6 days). These results indicated the enhanced effect of bioactive glass ions on key osteogenic markers important for the bone healing process.

Entities: CellLine Chemical Disease Gene Species

Mesh：

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Year: 2009 PMID： 19497391 PMCID： PMC6568011 DOI： 10.1016/j.actbio.2009.05.035

Source DB: PubMed Journal: Acta Biomater ISSN： 1742-7061 Impact factor: 8.947

45 in total

1. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution.

Authors: I D Xynos; A J Edgar; L D Buttery; L L Hench; J M Polak
Journal: J Biomed Mater Res Date: 2001-05

2. Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis.

Authors: I D Xynos; A J Edgar; L D Buttery; L L Hench; J M Polak
Journal: Biochem Biophys Res Commun Date: 2000-09-24 Impact factor: 3.575

3. Bioactive ceramics for periodontal treatment: comparative studies in the Patus monkey.

Authors: J Wilson; S B Low
Journal: J Appl Biomater Date: 1992

4. Bioactive glass coatings with hydroxyapatite and Bioglass particles on Ti-based implants. 1. Processing.

Authors: J M Gomez-Vega; E Saiz; A P Tomsia; G W Marshall; S J Marshall
Journal: Biomaterials Date: 2000-01 Impact factor: 12.479

5. Long-term changes of hydroxyapatite-coated dental implants.

Authors: I Baltag; K Watanabe; H Kusakari; N Taguchi; O Miyakawa; M Kobayashi; N Ito
Journal: J Biomed Mater Res Date: 2000

6. Glass-based coatings for titanium implant alloys.

Authors: J M Gomez-Vega; E Saiz; A P Tomsia
Journal: J Biomed Mater Res Date: 1999-09-15

7. Bioactive glass-mesoporous silica coatings on Ti6Al4V through enameling and triblock-copolymer-templated sol-gel processing.

Authors: J M Gomez-Vega; A Hozumi; E Saiz; A P Tomsia; H Sugimura; O Takai
Journal: J Biomed Mater Res Date: 2001-09-05

8. Phosphate is a specific signal for induction of osteopontin gene expression.

Authors: G R Beck; B Zerler; E Moran
Journal: Proc Natl Acad Sci U S A Date: 2000-07-18 Impact factor: 11.205

9. Activation of p42/44 and p38 mitogen-activated protein kinases by extracellular calcium-sensing receptor agonists induces mitogenic responses in the mouse osteoblastic MC3T3-E1 cell line.

Authors: T Yamaguchi; N Chattopadhyay; O Kifor; J L Sanders; E M Brown
Journal: Biochem Biophys Res Commun Date: 2000-12-20 Impact factor: 3.575

10. Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation In vitro: implications and applications for bone tissue engineering.

Authors: I D Xynos; M V Hukkanen; J J Batten; L D Buttery; L L Hench; J M Polak
Journal: Calcif Tissue Int Date: 2000-10 Impact factor: 4.333

26 in total

9. SiO2 and ZnO dopants in three-dimensionally printed tricalcium phosphate bone tissue engineering scaffolds enhance osteogenesis and angiogenesis in vivo.

Authors: Gary Fielding; Susmita Bose
Journal: Acta Biomater Date: 2013-07-18 Impact factor: 8.947

10. Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects.

Authors: Liangjiao Chen; Yanli Zhang; Jia Liu; Limin Wei; Bin Song; Longquan Shao
Journal: J Mater Sci Mater Med Date: 2016-01-22 Impact factor: 3.896

Enhanced osteocalcin expression by osteoblast-like cells (MC3T3-E1) exposed to bioactive coating glass (SiO2-CaO-P2O5-MgO-K2O-Na2O system) ions.

1. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution.

2. Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis.

3. Bioactive ceramics for periodontal treatment: comparative studies in the Patus monkey.

4. Bioactive glass coatings with hydroxyapatite and Bioglass particles on Ti-based implants. 1. Processing.

5. Long-term changes of hydroxyapatite-coated dental implants.

6. Glass-based coatings for titanium implant alloys.

7. Bioactive glass-mesoporous silica coatings on Ti6Al4V through enameling and triblock-copolymer-templated sol-gel processing.

8. Phosphate is a specific signal for induction of osteopontin gene expression.

9. Activation of p42/44 and p38 mitogen-activated protein kinases by extracellular calcium-sensing receptor agonists induces mitogenic responses in the mouse osteoblastic MC3T3-E1 cell line.

10. Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation In vitro: implications and applications for bone tissue engineering.

Review 1. Bioactive glasses as carriers for bioactive molecules and therapeutic drugs: a review.

2. Processing and characterization of innovative scaffolds for bone tissue engineering.

3. Comparative study of PCL-HAp and PCL-bioglass composite scaffolds for bone tissue engineering.

4. Role of Hydrogen and Nitrogen on the Surface Chemical Structure of Bioactive Amorphous Silicon Oxynitride Films.

Review 5. Nanotechnology approaches to improve dental implants.

6. Clay enriched silk biomaterials for bone formation.

Review 7. Perspectives on the role of nanotechnology in bone tissue engineering.

8. Si and Ca individually and combinatorially target enhanced MC3T3-E1 subclone 4 early osteogenic marker expression.

9. SiO2 and ZnO dopants in three-dimensionally printed tricalcium phosphate bone tissue engineering scaffolds enhance osteogenesis and angiogenesis in vivo.

10. Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects.