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Literature DB >> 19497049

Following the fate in vivo of COPI vesicles generated in vitro.

Christoph Rutz¹, Ayano Satoh, Paolo Ronchi, Britta Brügger, Graham Warren, Felix T Wieland.

Abstract

COPI vesicles are a class of transport carriers that function in the early secretory pathway. Their fate and function are still controversial. This includes their contribution to bidirectional transport within the Golgi apparatus and their role during cell division. Here we describe a method that should address several open questions about the fate and function of COPI vesicles in vivo. To this end, fluorescently labeled COPI vesicles were generated in vitro from isolated rat liver Golgi membranes, labeled with the fluorescent dyes Alexa-488 or Alexa-568. These vesicles appeared to be active and colocalized with endogenous Golgi membranes within 30 min after microinjection into mammalian cells. The COPI vesicle-derived labeled membrane proteins could be classified into two types that behaved like endogenous proteins after Brefeldin A treatment.

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Year: 2009 PMID： 19497049 PMCID： PMC4125364 DOI： 10.1111/j.1600-0854.2009.00934.x

Source DB: PubMed Journal: Traffic ISSN： 1398-9219 Impact factor: 6.215

56 in total

1. Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells.

Authors: Joachim Seemann; Marc Pypaert; Tomohiko Taguchi; Jorg Malsam; Graham Warren
Journal: Science Date: 2002-02-01 Impact factor: 47.728

2. The calcium-binding protein p54/NEFA is a novel luminal resident of medial Golgi cisternae that traffics independently of mannosidase II.

Authors: Valérie M Morel-Huaux; Marc Pypaert; Sandrine Wouters; Alan M Tartakoff; Ulrich Jurgan; Kris Gevaert; Pierre J Courtoy
Journal: Eur J Cell Biol Date: 2002-02 Impact factor: 4.492

Following the fate in vivo of COPI vesicles generated in vitro.

1. Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells.

2. The calcium-binding protein p54/NEFA is a novel luminal resident of medial Golgi cisternae that traffics independently of mannosidase II.

3. Rapid, endoplasmic reticulum-independent diffusion of the mitotic Golgi haze.

Review 4. The GGA proteins: adaptors on the move.

5. Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.

6. Functional reconstitution of COPI coat assembly and disassembly using chemically defined components.

7. Novel isotypic gamma/zeta subunits reveal three coatomer complexes in mammals.

Review 8. COP and clathrin-coated vesicle budding: different pathways, common approaches.

9. A monoclonal antibody against a 135-K Golgi membrane protein.

Review 10. Golgi inheritance: shaken but not stirred.

1. ER arrival sites for COPI vesicles localize to hotspots of membrane trafficking.

2. Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission.

3. CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.