| Literature DB >> 19495789 |
Federico Gómez Galindo1, Petr Dejmek, Krister Lundgren, Allan G Rasmusson, António Vicente, Thomas Moritz.
Abstract
Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.Entities:
Mesh:
Year: 2009 PMID: 19495789 DOI: 10.1007/s00425-009-0950-2
Source DB: PubMed Journal: Planta ISSN: 0032-0935 Impact factor: 4.116