Literature DB >> 19495789

Metabolomic evaluation of pulsed electric field-induced stress on potato tissue.

Federico Gómez Galindo1, Petr Dejmek, Krister Lundgren, Allan G Rasmusson, António Vicente, Thomas Moritz.   

Abstract

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

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Year:  2009        PMID: 19495789     DOI: 10.1007/s00425-009-0950-2

Source DB:  PubMed          Journal:  Planta        ISSN: 0032-0935            Impact factor:   4.116


  32 in total

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