BACKGROUND: Mast cells (MCs) play a central role in allergic reactions through high-affinity IgE receptor (FcepsilonRI)-mediated responses. Many attempts have been performed to investigate MC functions, though molecular bases of the intracellular signaling cascade through FcepsilonRI, especially in human MCs, remain scant and unexplored. METHODS: Human MCs were differentiated from CD34+ cells by culture with stem cell factor, IL-6 and IL-3. The differential phosphorylation profiles of protein tyrosine residues in the resulting MCs with or without FcepsilonRI aggregation were examined by two-dimensional gel electrophoresis. The candidate phosphoproteins of interest were picked, in-gel digested and mass spectrometry fingerprinted. RESULTS: Approximately 40 proteins in MCs were phosphorylated on their tyrosine residues in response to activation and some of them were identified. Particularly IL-31 receptor alpha, solute carrier family 39, syntaxin 5 and heterogeneous nuclear ribonucleoprotein are newly identified as phosphoproteins that are potentially involved in the MC signaling cascade through FcepsilonRI. CONCLUSION: Our present phosphoproteome data may provide the clue to understand the molecular mechanisms for the activation of human MCs. Copyright 2009 S. Karger AG, Basel.
BACKGROUND: Mast cells (MCs) play a central role in allergic reactions through high-affinity IgE receptor (FcepsilonRI)-mediated responses. Many attempts have been performed to investigate MC functions, though molecular bases of the intracellular signaling cascade through FcepsilonRI, especially in human MCs, remain scant and unexplored. METHODS:Human MCs were differentiated from CD34+ cells by culture with stem cell factor, IL-6 and IL-3. The differential phosphorylation profiles of protein tyrosine residues in the resulting MCs with or without FcepsilonRI aggregation were examined by two-dimensional gel electrophoresis. The candidate phosphoproteins of interest were picked, in-gel digested and mass spectrometry fingerprinted. RESULTS: Approximately 40 proteins in MCs were phosphorylated on their tyrosine residues in response to activation and some of them were identified. Particularly IL-31 receptor alpha, solute carrier family 39, syntaxin 5 and heterogeneous nuclear ribonucleoprotein are newly identified as phosphoproteins that are potentially involved in the MC signaling cascade through FcepsilonRI. CONCLUSION: Our present phosphoproteome data may provide the clue to understand the molecular mechanisms for the activation of human MCs. Copyright 2009 S. Karger AG, Basel.
Authors: Nicholas J Shubin; Veronika A Glukhova; Morgan Clauson; Phuong Truong; Magnus Abrink; Gunnar Pejler; Nathan J White; Gail H Deutsch; Stephen R Reeves; Tomas Vaisar; Richard G James; Adrian M Piliponsky Journal: J Allergy Clin Immunol Date: 2016-05-13 Impact factor: 10.793
Authors: Lau F Larsen; Nanna Juel-Berg; Anker Hansen; Kirsten S Hansen; E N Clare Mills; Ronald van Ree; Madeleine Rådinger; Lars K Poulsen; Bettina M Jensen Journal: Immun Inflamm Dis Date: 2018-07-10