Literature DB >> 194917

Rapid biochemical tests for characterization of the Mycoplasmatales.

J M Bradbury.   

Abstract

Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.

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Year:  1977        PMID: 194917      PMCID: PMC274645          DOI: 10.1128/jcm.5.5.531-534.1977

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  5 in total

1.  Simple method for immunofluorescent identification of mycoplasma colonies.

Authors:  J M Bradbury; C A Oriel; F T Jordan
Journal:  J Clin Microbiol       Date:  1976-04       Impact factor: 5.948

2.  Bovine mycoplasmas: cultural and biochemical studies. II.

Authors:  H Erno; L Stipkovits
Journal:  Acta Vet Scand       Date:  1973       Impact factor: 1.695

3.  Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma.

Authors:  J Fabricant; E A Freundt
Journal:  Ann N Y Acad Sci       Date:  1967-07-28       Impact factor: 5.691

4.  Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone.

Authors:  T J Jacks; H W Kircher
Journal:  Anal Biochem       Date:  1967-11       Impact factor: 3.365

5.  A rapid method for identifying bacterial enzymes.

Authors:  J L Maddocks; M J Greenan
Journal:  J Clin Pathol       Date:  1975-08       Impact factor: 3.411

  5 in total
  8 in total

1.  Rapid biochemical tests for the identification of groups A, B, C, F, and G streptococci from throat cultures.

Authors:  M Slifkin; G M Gil
Journal:  J Clin Microbiol       Date:  1983-07       Impact factor: 5.948

2.  Rapid detection of yeast enzymes by using 4-methylumbelliferyl substrates.

Authors:  D G Bobey; G M Ederer
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948

3.  Rapid identification of Branhamella catarrhalis with 4-methylumbelliferyl butyrate.

Authors:  M Vaneechoutte; G Verschraegen; G Claeys; P Flamen
Journal:  J Clin Microbiol       Date:  1988-06       Impact factor: 5.948

4.  Detection of mixed Mycoplasma species.

Authors:  J M Bradbury; M McClenaghan
Journal:  J Clin Microbiol       Date:  1982-08       Impact factor: 5.948

5.  Pathogenicity and cytadherence of Mycoplasma imitans in chicken and duck embryo tracheal organ cultures.

Authors:  O M Abdul-Wahab; G Ross; J M Bradbury
Journal:  Infect Immun       Date:  1996-02       Impact factor: 3.441

6.  Typing of strains from a single-source outbreak of Pseudomonas pickettii.

Authors:  W J Dimech; A G Hellyar; M Kotiw; D Marcon; S Ellis; M Carson
Journal:  J Clin Microbiol       Date:  1993-11       Impact factor: 5.948

Review 7.  Implementing best practices and validation of cryopreservation techniques for microorganisms.

Authors:  David Smith; Matthew Ryan
Journal:  ScientificWorldJournal       Date:  2012-05-02

8.  Mycoplasma gallisepticum infection in the grey partridge Perdix perdix: outbreak description, histopathology, biochemistry and antioxidant parameters.

Authors:  Frantisek Vitula; Lucie Peckova; Hana Bandouchova; Miroslav Pohanka; Ladislav Novotny; David Jira; Jiri Kral; Karel Ondracek; Jitka Osickova; Dagmar Zendulkova; Katerina Rosenbergova; Frantisek Treml; Jiri Pikula
Journal:  BMC Vet Res       Date:  2011-07-08       Impact factor: 2.741

  8 in total

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