OBJECTIVE: Human beta-defensin-2 (BD-2) is a positive ion antimicrobial peptide. We investigated the effects of intestinal ischaemia/reperfusion (II/R) on rat BD-2 mRNA and protein expressions in rat lung to address the potential role of BD-2 in acute lung injury (ALI) induced by II/R. METHODS: Rats were randomly divided into two groups (n=36 each). (i) Sham control and (ii) II/R group (1h superior mesenteric artery clamping, followed by reperfusion of different durations). In II/R group, 6 animals were sacrificed at 0min, 15min, 30min, 60min, 3h and 6h after reperfusion, and serum, lung tissue and bronchoalveolar lavage fluid were harvested. Samples were taken at the corresponding time points in the sham group. Lung histological changes were observed under microscope and the pulmonary permeability index (PPI) was calculated. The lung tissue levels of TNFalpha were detected by ELISA. BD-2 mRNA and protein expressions were examined by RT-PCR and western blotting techniques, respectively. RESULTS: ALI induced by II/R was confirmed by pathological examination and significantly increased PPI (P<0.05 or 0.01). II/R significantly increased the lung TNFalpha levels and upregulated the expressions of BD-2 mRNA and protein expressions (P<0.05 or 0.01). BD-2 mRNA expression was significantly positively correlated to the lung TNFalpha level (r=0.823, P<0.01) and negatively correlated to PPI (r=-0.615, P<0.05). CONCLUSION: II/R can upregulate BD-2 mRNA and protein expressions in rat lung. BD-2 could be an innate protective factor against II/R-induced lung injury.
OBJECTIVE:Humanbeta-defensin-2 (BD-2) is a positive ion antimicrobial peptide. We investigated the effects of intestinal ischaemia/reperfusion (II/R) on ratBD-2 mRNA and protein expressions in rat lung to address the potential role of BD-2 in acute lung injury (ALI) induced by II/R. METHODS:Rats were randomly divided into two groups (n=36 each). (i) Sham control and (ii) II/R group (1h superior mesenteric artery clamping, followed by reperfusion of different durations). In II/R group, 6 animals were sacrificed at 0min, 15min, 30min, 60min, 3h and 6h after reperfusion, and serum, lung tissue and bronchoalveolar lavage fluid were harvested. Samples were taken at the corresponding time points in the sham group. Lung histological changes were observed under microscope and the pulmonary permeability index (PPI) was calculated. The lung tissue levels of TNFalpha were detected by ELISA. BD-2 mRNA and protein expressions were examined by RT-PCR and western blotting techniques, respectively. RESULTS: ALI induced by II/R was confirmed by pathological examination and significantly increased PPI (P<0.05 or 0.01). II/R significantly increased the lung TNFalpha levels and upregulated the expressions of BD-2 mRNA and protein expressions (P<0.05 or 0.01). BD-2 mRNA expression was significantly positively correlated to the lung TNFalpha level (r=0.823, P<0.01) and negatively correlated to PPI (r=-0.615, P<0.05). CONCLUSION: II/R can upregulate BD-2 mRNA and protein expressions in rat lung. BD-2 could be an innate protective factor against II/R-induced lung injury.
Authors: Aric L Gregson; Aki Hoji; Patil Injean; Steven T Poynter; Claudia Briones; Vyacheslav Palchevskiy; S Sam Weigt; Michael Y Shino; Ariss Derhovanessian; David Sayah; Rajan Saggar; David Ross; Abbas Ardehali; Joseph P Lynch; John A Belperio Journal: Am J Respir Crit Care Med Date: 2015-12-15 Impact factor: 21.405
Authors: Valentina Volynets; Andreas Rings; Gyöngyi Bárdos; Maureen J Ostaff; Jan Wehkamp; Stephan C Bischoff Journal: Tissue Barriers Date: 2016-07-08
Authors: Felix S Meier-Stephenson; Vanessa C Meier-Stephenson; Michael D Carter; Autumn R Meek; Yanfei Wang; Luzhe Pan; Qiangwei Chen; Sheila Jacobo; Fan Wu; Erhu Lu; Gordon A Simms; Laural Fisher; Alaina J McGrath; Virgil Fermo; Christopher J Barden; Harman D S Clair; Todd N Galloway; Arun Yadav; Valérie Campágna-Slater; Mark Hadden; Mark Reed; Marcia Taylor; Brendan Kelly; Elena Diez-Cecilia; Igri Kolaj; Clarissa Santos; Imindu Liyanage; Braden Sweeting; Paul Stafford; Robert Boudreau; G Andrew Reid; Ryan S Noyce; Leanne Stevens; Agnieszka Staniszewski; Hong Zhang; Mamidanna R V S Murty; Pascale Lemaire; Solenne Chardonnet; Christopher D Richardson; Valérie Gabelica; Edwin DePauw; Richard Brown; Sultan Darvesh; Ottavio Arancio; Donald F Weaver Journal: Alzheimers Dement (N Y) Date: 2022-04-06