PURPOSE: The aim of this study was to evaluate the effect of three denture hygiene methods against different microbial biofilms formed on acrylic resin specimens. MATERIALS AND METHODS: The set (sterile stainless steel basket and specimens) was contaminated (37 degrees C for 48 hours) by a microbial inoculum with 10(6) colony-forming units (CFU)/ml (standard strains: Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, and Enterococcus faecalis; field strains: S. mutans, C. albicans, C. glabrata, and C. tropicalis). After inoculation, specimens were cleansed by the following methods: (1) chemical: immersion in an alkaline peroxide solution (Bonyplus tablets) for 5 minutes; (2) mechanical: brushing with a dentifrice for removable prostheses (Dentu Creme) for 20 seconds; and (3) a combination of chemical and mechanical methods. Specimens were applied onto a Petri plate with appropriate culture medium for 10 minutes. Afterward, the specimens were removed and the plates incubated at 37 degrees C for 48 hours. RESULTS: Chemical, mechanical, and combination methods showed no significant difference in the reduction of CFU for S. aureus, S. mutans (ATCC and field strain), and P. aeruginosa. Mechanical and combination methods were similar and more effective than the chemical method for E. faecalis, C. albicans (ATCC and field strain), and C. glabrata. The combination method was better than the chemical method for E. coli and C. tropicalis, and the mechanical method showed intermediate results. CONCLUSION: The three denture hygiene methods showed different effects depending on the type of microbial biofilms formed on acrylic base resin specimens.
PURPOSE: The aim of this study was to evaluate the effect of three denture hygiene methods against different microbial biofilms formed on acrylic resin specimens. MATERIALS AND METHODS: The set (sterile stainless steel basket and specimens) was contaminated (37 degrees C for 48 hours) by a microbial inoculum with 10(6) colony-forming units (CFU)/ml (standard strains: Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, and Enterococcus faecalis; field strains: S. mutans, C. albicans, C. glabrata, and C. tropicalis). After inoculation, specimens were cleansed by the following methods: (1) chemical: immersion in an alkaline peroxide solution (Bonyplus tablets) for 5 minutes; (2) mechanical: brushing with a dentifrice for removable prostheses (Dentu Creme) for 20 seconds; and (3) a combination of chemical and mechanical methods. Specimens were applied onto a Petri plate with appropriate culture medium for 10 minutes. Afterward, the specimens were removed and the plates incubated at 37 degrees C for 48 hours. RESULTS: Chemical, mechanical, and combination methods showed no significant difference in the reduction of CFU for S. aureus, S. mutans (ATCC and field strain), and P. aeruginosa. Mechanical and combination methods were similar and more effective than the chemical method for E. faecalis, C. albicans (ATCC and field strain), and C. glabrata. The combination method was better than the chemical method for E. coli and C. tropicalis, and the mechanical method showed intermediate results. CONCLUSION: The three denture hygiene methods showed different effects depending on the type of microbial biofilms formed on acrylic base resin specimens.
Authors: Cláudia Helena Silva-Lovato; Bart de Wever; Els Adriaens; Helena de Freitas Oliveira Paranhos; Evandro Watanabe; Maria Xavier Pisani; Rafael Freitas de Souza; Isabel Yoko Ito Journal: J Appl Oral Sci Date: 2010-12 Impact factor: 2.698
Authors: Sílvia Carneiro de Lucena-Ferreira; Antonio Pedro Ricomini-Filho; Wander José da Silva; Jaime Aparecido Cury; Altair Antoninha Del Bel Cury Journal: Clin Oral Investig Date: 2014-03-04 Impact factor: 3.573
Authors: Laura Susana Acosta-Torres; Irasema Mendieta; Rosa Elvira Nuñez-Anita; Marcos Cajero-Juárez; Víctor M Castaño Journal: Int J Nanomedicine Date: 2012-09-03