R C Anderson1, N A Krueger, J A Byrd, R B Harvey, T R Callaway, T S Edrington, D J Nisbet. 1. United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, 2881 F&B Road, College Station, Texas, USA. Robin.Anderson@ars.usda.gov
Abstract
AIMS: To determine if the purported deaminase inhibitors diphenyliodonium chloride (DIC) and thymol reduce the growth and survivability of Campylobacter. METHODS AND RESULTS: Growth rates of Campylobacter jejuni and Camp. coli were reduced compared to unsupplemented controls during culture in Muellar-Hinton broth supplemented with 0.25 micromol DIC or thymol ml(-1) but not with 0.01 micromol monensin ml(-1) or 1% ethanol. Recovery of Camp. jejuni and Camp. coli was reduced >5 log(10) CFU from controls after 24 h pure culture in Bolton broth supplemented with 0.25 or 1.0 micromol DIC ml(-1) or with 1.0 micromol thymol ml(-1). Similarly, each test Campylobacter strain was reduced >3 log(10) CFU from controls after 24 h mixed culture with porcine faecal microbes in Bolton broth supplemented with 0.25 or 1.0 micromol DIC ml(-1) or with 1.0 micromol thymol ml(-1). Treatments with 0.25 micromol thymol ml(-1), 0.01 micromol monensin ml(-1) or 1% ethanol were less effective. Ammonia production during culture or incubation of cell lysates was reduced by 0.25 or 1.0 micromol DIC ml(-1) but only intermittently reduced, if at all, by the other treatments. CONCLUSIONS: Diphenyliodonium chloride and thymol reduced growth, survivability and ammonia production of Camp. jejuni and Camp. coli. SIGNIFICANCE AND IMPACT OF THE STUDY: Results suggest a potential physiological characteristic that may be exploited to develop interventions.
AIMS: To determine if the purported deaminase inhibitors diphenyliodonium chloride (DIC) and thymol reduce the growth and survivability of Campylobacter. METHODS AND RESULTS: Growth rates of Campylobacter jejuni and Camp. coli were reduced compared to unsupplemented controls during culture in Muellar-Hinton broth supplemented with 0.25 micromol DIC or thymol ml(-1) but not with 0.01 micromol monensin ml(-1) or 1% ethanol. Recovery of Camp. jejuni and Camp. coli was reduced >5 log(10) CFU from controls after 24 h pure culture in Bolton broth supplemented with 0.25 or 1.0 micromol DIC ml(-1) or with 1.0 micromol thymol ml(-1). Similarly, each test Campylobacter strain was reduced >3 log(10) CFU from controls after 24 h mixed culture with porcine faecal microbes in Bolton broth supplemented with 0.25 or 1.0 micromol DIC ml(-1) or with 1.0 micromol thymol ml(-1). Treatments with 0.25 micromol thymol ml(-1), 0.01 micromol monensin ml(-1) or 1% ethanol were less effective. Ammonia production during culture or incubation of cell lysates was reduced by 0.25 or 1.0 micromol DIC ml(-1) but only intermittently reduced, if at all, by the other treatments. CONCLUSIONS:Diphenyliodonium chloride and thymol reduced growth, survivability and ammonia production of Camp. jejuni and Camp. coli. SIGNIFICANCE AND IMPACT OF THE STUDY: Results suggest a potential physiological characteristic that may be exploited to develop interventions.
Authors: R C Anderson; M D Flythe; N A Krueger; T R Callaway; T S Edrington; R B Harvey; D J Nisbet Journal: Folia Microbiol (Praha) Date: 2010-08-03 Impact factor: 2.099
Authors: Robin C Anderson; Maša Vodovnik; Byeng R Min; William E Pinchak; Nathan A Krueger; Roger B Harvey; David J Nisbet Journal: Folia Microbiol (Praha) Date: 2012-04-20 Impact factor: 2.099
Authors: Gizem Levent; Robin C Anderson; Branko Petrujkić; Toni L Poole; Haiqi He; Kenneth J Genovese; Michael E Hume; Ross C Beier; Roger B Harvey; David J Nisbet Journal: Microorganisms Date: 2021-04-16