Y Cheng1, X Song, Y Qin, Y Qu. 1. State Key laboratory of Microbial Technology, National Glycoengineering Reserch Center, Shandong University, Jinan, China.
Abstract
AIMS: Improvement of cellulase production of Penicillium decumbens by genome shuffling of an industrial catabolite-repression-resistant strain JU-A10 with its mutants. METHODS AND RESULTS: After two rounds of genome shuffling, three fusants, GS2-15, GS2-21 and GS2-22, were obtained, showing 100%, 109% and 94% increase in FPase activity than JU-A10 respectively. The cellulase production of the fusants on various substrates, such as corn stover, wheat straw, bagasse and the corncob residue, was studied. The maximum productivities of GS2-15, GS2-21 and GS2-22 were 92.15, 102.63 and 92.35 FPU l(-1) h(-1) on the corncob residue at 44 h respectively, which were 117%, 142% and 118% higher than that of JU-A10 (42.44 FPU l(-1) h(-1), at 90 h). The improvements of the fusants were possibly because of their enhanced growth rates, earlier cellulase synthesis and higher secretion of extracellular proteins. CONCLUSIONS: The fusants obtained after genome shuffling could produce abundant cellulase much earlier, and they could be potential candidates for bioconversion process. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report on the improvement of cellulase production in fungi by genome shuffling, and this is a good technique to improve other important phenotypes in fungi.
AIMS: Improvement of cellulase production of Penicillium decumbens by genome shuffling of an industrial catabolite-repression-resistant strain JU-A10 with its mutants. METHODS AND RESULTS: After two rounds of genome shuffling, three fusants, GS2-15, GS2-21 and GS2-22, were obtained, showing 100%, 109% and 94% increase in FPase activity than JU-A10 respectively. The cellulase production of the fusants on various substrates, such as corn stover, wheat straw, bagasse and the corncob residue, was studied. The maximum productivities of GS2-15, GS2-21 and GS2-22 were 92.15, 102.63 and 92.35 FPU l(-1) h(-1) on the corncob residue at 44 h respectively, which were 117%, 142% and 118% higher than that of JU-A10 (42.44 FPU l(-1) h(-1), at 90 h). The improvements of the fusants were possibly because of their enhanced growth rates, earlier cellulase synthesis and higher secretion of extracellular proteins. CONCLUSIONS: The fusants obtained after genome shuffling could produce abundant cellulase much earlier, and they could be potential candidates for bioconversion process. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report on the improvement of cellulase production in fungi by genome shuffling, and this is a good technique to improve other important phenotypes in fungi.