| Literature DB >> 19484295 |
Sukhvinder Kaur1, Majid Rasool Kamli, Arif Ali.
Abstract
A polymerase chain reaction (PCR) approach was used to assess the occurrence and diversity of arsenate reductase gene (arsC gene) in arsenic-resistant environmental E. coli strains. For this purpose, two different sets of primers were designed for the specific amplification of approximately 370-bp fragments from the arsC gene. These primers were used to screen a collection of 25 environmental arsenic-resistant strains isolated from different geographical regions of India, as well as Bangladesh. The PCR results showed that 17 out of the 25 environmental isolates (68%) contained a gene related to the arsC family. Phylogenetic analysis of the protein sequences deduced from the amplicons indicated a prevalence of arsC genes in the isolated strains. A significant divergence in the DNA sequence was found in the arsC genes among As-resistant environmental E. coli strains from this study, and arsenic resistance, a genetic character, arose from a common ancestral background.Entities:
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Year: 2009 PMID： 19484295 DOI： 10.1007/s00284-009-9432-9
Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188