Yukihiro Tatekawa1, Yoshito Ikada, Hiroaki Komuro, Michio Kaneko. 1. Department of Pediatric Surgery, Graduate School of Comprehensive Human Sciences and University Hospital, University of Tsukuba, Tsukuba, Japan. tatekawa3966@s5.dion.ne.jp
Abstract
BACKGROUND: We investigated epithelialization and newly formed cartilage in an artificial trachea constructed using a bioabsorbable copolymer. MATERIALS AND METHODS: Fifteen male Japanese white rabbits (2.5-2.8 kg) were divided into three groups. A full-thickness anterior defect (4 mm x 10 mm) was created in the trachea. The defect was implanted with one of the following bioabsorbable copolymers: caprolactone-lactide copolymer sponge sheet reinforced with poly(glycolic acid) fiber mesh (Cop) (n = 6, group A), Cop-incorporating gelatin hydrogel (n = 4, group B), and Cop-incorporating gelatin hydrogel with 100 microg of basic fibroblast growth factor (n = 5, group C). Each trachea was reinforced with an external nondegradable polymer stent. Three rabbits in each group were sacrificed at 1, 3, and 6 mo postoperatively and the trachea was evaluated histologically; other animals were sacrificed up to 12 mo postoperatively. RESULTS: In groups A, B, and C there were two, one, and one postoperative deaths, respectively. In group A, epithelialization was recognized from 1 mo to 12 mo postoperatively, but no new cartilage was formed during the 12 mo following implantation. In group B, epithelialization was recognized 3 and 6 mo postoperatively, and new cartilage was detected at 6 mo after the operation. In group C, newly formed cartilage and epithelialization were observed 3, 6, and even 12 mo postoperatively. Furthermore, neovascularization was observed in groups B and C. CONCLUSIONS: A bioabsorbable copolymer incorporating gelatin hydrogel induces tracheal epithelialization and formation of cartilage and vessels in tracheal defects, and could be available for clinical use in children. Copyright 2010 Elsevier Inc. All rights reserved.
BACKGROUND: We investigated epithelialization and newly formed cartilage in an artificial trachea constructed using a bioabsorbable copolymer. MATERIALS AND METHODS: Fifteen male Japanese white rabbits (2.5-2.8 kg) were divided into three groups. A full-thickness anterior defect (4 mm x 10 mm) was created in the trachea. The defect was implanted with one of the following bioabsorbable copolymers: caprolactone-lactide copolymer sponge sheet reinforced with poly(glycolic acid) fiber mesh (Cop) (n = 6, group A), Cop-incorporating gelatin hydrogel (n = 4, group B), and Cop-incorporating gelatin hydrogel with 100 microg of basic fibroblast growth factor (n = 5, group C). Each trachea was reinforced with an external nondegradable polymer stent. Three rabbits in each group were sacrificed at 1, 3, and 6 mo postoperatively and the trachea was evaluated histologically; other animals were sacrificed up to 12 mo postoperatively. RESULTS: In groups A, B, and C there were two, one, and one postoperative deaths, respectively. In group A, epithelialization was recognized from 1 mo to 12 mo postoperatively, but no new cartilage was formed during the 12 mo following implantation. In group B, epithelialization was recognized 3 and 6 mo postoperatively, and new cartilage was detected at 6 mo after the operation. In group C, newly formed cartilage and epithelialization were observed 3, 6, and even 12 mo postoperatively. Furthermore, neovascularization was observed in groups B and C. CONCLUSIONS: A bioabsorbable copolymer incorporating gelatin hydrogel induces tracheal epithelialization and formation of cartilage and vessels in tracheal defects, and could be available for clinical use in children. Copyright 2010 Elsevier Inc. All rights reserved.