| Literature DB >> 19481426 |
Jiong Liu1, Shengrong Guo, Zonghai Li, Li Liu, Jianren Gu.
Abstract
A novel gene carrier, stearyl protamine (SP) was synthesized by the reaction of natural protamine with an N-hydroxysuccinimide (NHS) ester of stearic acid. This SP was characterized by FT-IR, stearyl content measurement and dynamic light scattering (DLS). Then, the SP was evaluated as a gene delivery carrier. The SP with higher stearyl group content (SP3) can self-assemble into aggregates with an average diameter of ca. 110 nm in water and has relatively higher in vitro gene transfection efficiency than protamine and SP with lower stearyl group content. A possible mechanism is proposed for the formation of complex of SP/DNA. The SP also shows a low cytotoxicity similar to protamine.Entities:
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Year: 2009 PMID： 19481426 DOI： 10.1016/j.colsurfb.2009.04.026
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268